畜牧与兽医2024，Vol.56Issue(9)：48-55,8.
新西兰兔下丘脑qPCR内参基因的筛选和验证
Selection and validation of qPCR reference genes in hypothalamus of New Zealand rabbits
摘要
Abstract
We selected seven reference genes as candidates,including GAPDH,ACTB,B2M,YWHAZ,HPRT1,RPL4,and TBP,to find a suitable gene for the hypothalamus of New Zealand white rabbits.The gene expression levels in the hypothalamus of New Zealand white rab-bits of different ages,genders,and estrus states were detected by qPCR.Then,RefFinder was used to evaluate the stability of the reference gene expression by combining the analysis results of ΔCt,GeNorm,NormFinder and BestKeper.Next,ER1,PGR and NPVF were used to verify the stability of the candidate reference genes and the reliability of the analysis results.The results showed that YWHAZ and GAPDH were the most stable hypothalamic reference genes for the female rabbits,while YWHAZ and TBP were recommended for the male rabbits;and HPRT1 and YWHAZ were good for the rabbits in different estrus cycles.When different reference genes were used for normalization,the expression level of the target gene would change significantly depending on the physiological state of the sample,underlining the importance of a priori validation of reference genes for qPCR studies.关键词
新西兰白兔/下丘脑/内参基因/稳定性评价和验证Key words
New Zealand white rabbit/hypothalamus/reference genes/stability evaluation and verification分类
农业科技引用本文复制引用
姜幸慧,陈晶,张洺瑞,邱水玲,甘乾福..新西兰兔下丘脑qPCR内参基因的筛选和验证[J].畜牧与兽医,2024,56(9):48-55,8.基金项目
福建省中青年教师教育科研项目(JAT220070) （JAT220070）
福建省科技计划项目[高校产学合作项目(2023N5004),星火项目(2023S0007,2023S0015,2023S0054),对外合作项目(2023I1009)] （2023N5004）
福建农林大学农村振兴服务团队-草食性动物产业服务团队项目(11899170139) （11899170139）
