畜牧与兽医2024，Vol.56Issue(9)：61-69,9.
3株鸭腺病毒3型的分离鉴定与主要结构蛋白的生物信息学分析
Isolation and identification of three duck adenovirus type 3 strains and bioinformatic analysis of four major structural proteins of the strains
摘要
Abstract
This study aimed to isolate the Guangdong epidemic strain of duck adenovirus 3(DAdV-3),and to predict and analyze the physicochemical characteristics,structure,and function of its four major structural proteins(Hexon,Penton,Fiber1,and Fiber2).Suspec-ted DAdV-3 infected duck and goose liver tissue samples were collected and subjected to virus isolation using chicken hepatoma cells(LMH).The major structural proteins(see above)of the four viral isolates were cloned,sequenced,and assembled.The nucleotide se-quences of the four proteins were analyzed using the MEGA 11 software so as to perform amino acid sequence analysis and to construct phylo-genetic trees.The physicochemical properties and secondary structures of the four proteins were analyzed using the Expasy-ProtParam and SOPMA software.The tertiary structures of the proteins were predicted using the AlphaFold2 software,and their linear B-cell epitopes were predicted.The results showed that three DAdV-3 virus strains were successfully isolated and were named XZD-2021,FRK-2021,and 825-1-2021,respectively.The four proteins of the three DAdV-3 strains were in the same branch as other DAdV-3 isolates in GenBank.The Hexon protein was closer to Goose adenovirus type 4,the Penton protein was closer to type D avian adenovirus,and the Fiber1 and Fiber2 proteins were closer to Pigeon adenovirus type 1.All four proteins were acidic hydrophilic proteins without signal peptides.The Hexon,Fi-ber1,and Fiber2 proteins were composed of random coils,while the Penton protein was predominantly of α-helix.The tertiary structure pre-dictions were consistent with the secondary structure results.The linear B-cell epitope prediction revealed that the Hexon protein had the most(11)linear B-cell epitopes,followed by the Penton and Fiber2 proteins(6 each),and the Fiber1 protein(5).However,Fiber1 had the most surface antigen epitopes with higher lengths than Fiber2,which was primarily located in the head domain responsible for binding to host cell receptors.In summary,in this study,three DAdV-3 strains were successfully isolated and the genetic evolution,secondary struc-ture,and tertiary structure of their four major structural proteins were analyzed.The results provided a foundation for further research.关键词
鸭腺病毒/分离鉴定/遗传进化分析/生物信息学Key words
duck adenovirus/isolation and identification/genetic evolution analysis/bioinformatics分类
畜牧业引用本文复制引用
陈作鑫,梅堃,姜雪芹,刘梦凡,赵梓桥,魏无缺,李树稳,张雪莲,刘兆洁,黄淑坚..3株鸭腺病毒3型的分离鉴定与主要结构蛋白的生物信息学分析[J].畜牧与兽医,2024,56(9):61-69,9.基金项目
广东省重要新发水禽疫病防控共性关键技术集成与应用研究项目(2020ZDZX1004) （2020ZDZX1004）
广东省水禽产业技术体系创新团队项目(2023KJ137) （2023KJ137）
