血清4型禽腺病毒的分离鉴定及致病性分析OA北大核心CSTPCD

Fowl adenovirus(FAdV)was isolated and identified from the infected chickens,in order to provide reference for clinical diag-nosis and disease control.The positive samples were inoculated with SPF chicken embryos and passed down to the next generation.The virus was identified as avian serum type 4 adenovirus by PCR detection,gene sequence analysis and animal regression test.The results showed that pure FAdV was obtained by chicken embryo passage and virus specific detection,and was named HB2306.The Hexon gene sequence analy-sis showed that the HB2306 strain belonged to the serum type 4 branch,and its nucleotide sequence homology with FAdV-4 isolates from both domestic and international sources was 98.2%-99.9%.The amino acid sequence similarity between HB2306 and other highly pathogen-ic type 4 was 97.9%-99.8%.The clinical pathological changes of the tested animals showed that HB2306 strain caused typical pathological changes,such as pericardial effusion,yellow swelling of liver,congestion and hemorrhage,kidney hemorrhage and swelling after injection into the chest muscle at the dose of 104.5 EID50/strain.The clinical symptoms were similar to those of other popular highly pathogenic strains,and the mortality rate was 100%.In conclusion,in this study,a highly virulent strain was successfully isolated and identified,which en-riched the virus reservoir and provided data for the prevalence and for the comprehensive prevention and control of FAdV.