畜牧与兽医2024，Vol.56Issue(9)：96-103,8.
宠物用丹参咀嚼片制备工艺研究及质量标准建立
Research on the preparation of chewable Salvia miltiorrhiza tablet for pets and on establishment of related quality standard
摘要
Abstract
This study was to determine the preparation process of chewable Salvia miltiorrhiza tablet for pets and to establish the quality standard for the preparation.In the test,the preparation process was determined by observing and examining the wet grain accessories,granu-lation process and tablet pressing process.Thin layer chromatography and high performance liquid chromatography(HPLC)were used to car-ry out qualitative and quantitative studies on Tanshinone IIA and Salvianolic acid B in the chewable tablets,and to establish a quality stand-ard for the prepared tablet.The results showed that,in the tablet,the Salvia miltiorrhiza spray powder was mixed evenly.The mixing time was 5 min,100 r/min,with 10%starch slurry as adhesive,at drying temperature 40-50℃,with 0.3%magnesium stearate as lubricant;and the compression rate was 10-35 r/min.The identification results showed that Tanshinone IIA had clear spots,good separation and strong specificity.The high performance liquid chromatography results showed that Salvianolic acid B had a good linear relationship from 8.0 to 256.0 μg/mL(R2=0.999 9),system applicability,specificity,precision,stability,repeatability,and system durability,with a sample re-covery of 95.0%-105.0%.For Salvianolic acid B in the six samples,the content limit was set at 11.484 1 mg/g.It showed that the prepara-tion process of the chewable tablets for pets determined in this test was stable and feasible,and that the established thin layer identification method and HPLC determination method were helpful for the quality control of preparing chewable Salvia miltiorrhiza tablet for pets.关键词
宠物用丹参咀嚼片/制备工艺/薄层色谱法/高效液相色谱法/质量标准Key words
chewable tablets for pets/preparation process/thin layer chromatography/high performance liquid chromatography/quality standard分类
农业科技引用本文复制引用
郭沛,吕肖静,秦志华,刘家国..宠物用丹参咀嚼片制备工艺研究及质量标准建立[J].畜牧与兽医,2024,56(9):96-103,8.基金项目
国家重点研发计划项目(2022YFD1801102) （2022YFD1801102）
山东省重点研发计划项目(2019GNC106042) （2019GNC106042）
