岩矿测试2024，Vol.43Issue(4)：582-591,10.DOI:10.15898/j.ykcs.202402060015
石灰土易提取球囊霉素相关土壤蛋白的实验条件优化
Optimization of Extraction Method for Easily Extractable Glomalin-Related Soil Protein from Calcareous Soil
摘要
Abstract
Glomalin-related soil protein(GRSP)secreted by arbuscular mycorrhizal fungi is widely distributed worldwide,has stable properties and is not easily decomposed,which is an important component of soil organic matter.Extracting high quality GRSP is important for in-depth research on organic carbon sinks in calcareous soil from karst areas.However,in previous studies,the mechanism of GRSP in organic matter could not be further studied due to low extraction yield,insufficient extraction and non-specific products.Therefore,the high extraction amount of GRSP is of great significance to explore the formation and stabilization mechanism of organic matter in calcareous soil.This experiment selected four types of calcareous soil from karst areas:black,brown,yellow and red calcareous soil.By using orthogonal experiments of temperature and time,the optimal extraction conditions for easily extractable glomalin-related soil protein(EE-GRSP)related to particulate organic matter(POM)and mineral-associated organic matter(MAOM)in calcareous soil were selected.The experimental results showed that the highest extraction amount of EE-GRSP was achieved when POM and MAOM were extracted at 123 ℃ and 80min.After application into the four types of calcareous soil,the EE-GRSP contents increased from 4.6％to 34.2％.The BRIEF REPORT is available for this paper at http://www.ykcs.ac.cn/en/article/doi/10.15898/j.ykcs.yk202402060015.关键词
易提取球囊霉素相关土壤蛋白/提取条件优化/颗粒态有机质/矿物结合态有机质Key words
easily extractable glomalin-related soil protein/optimization of extraction method/particulate organic matter/mineral-associated organic matter分类
农业科技引用本文复制引用
蒋利,康志强,梁月明,吴泽燕,涂纯,李强..石灰土易提取球囊霉素相关土壤蛋白的实验条件优化[J].岩矿测试,2024,43(4):582-591,10.基金项目
国家自然科学基金项目(42172341) （42172341）
广西重点研发计划项目(桂科AB21196050) （桂科AB21196050）
中国地质科学院岩溶地质研究所基本科研业务费项目(2023020) （2023020）
