云南化工2024，Vol.51Issue(8)：56-61,6.DOI:10.3969/j.issn.1004-275X.2024.08.12
雌二醇降解菌的筛选鉴定及降解特性研究
Screening and Characterization of Estradiol-degrading Bacteria and Their Degradation Properties
摘要
Abstract
In order to achieve the biodegradation of estrogens in tobacco sheet wastewater,this study selected a dominant degrading strain from the sludge at the influent of tobacco wastewater,using high concentrations of estradiol(Estradiol,E2)as the sole carbon source.The strain ap-peared as creamy white,round,and shiny colonies on E2 agar plates,with a diameter of approximately 0.1-0.5 mm,and was identified as a Gram-negative bacterium through 16S rRNA analysis,designated as Pseudomonas sp.CO03.Based on single-factor experiments,with the degradation rate of E2 as the response value,the temperature,pH,and substrate concentration during the degradation process were optimized u-sing the Box-Benhnken experimental design and response surface analysis method.The results indicated that temperature had the greatest im-pact on the E2 degradation rate,followed by E2 concentration,with pH having the least effect.The optimal conditions for degrading E2 were determined to be:a temperature of 37 ℃,a pH value of 7.0,and an E2 concentration of 5.5 mg/L,under which the degradation rate of E2 was measured to be 81.67％.When this strain was applied to the degradation of E2 in tobacco concentrate and at the influent of tobacco wastewater,the degradation rates of E2 after 7 days were 31.21％and 53.14％,respectively.The highly efficient degrading strain obtained from this study has a strong ability and high efficiency for E2 degradation,which can be used for the biodegradation of estrogens in tobacco sheet wastewater,and is of significant importance for the degradation of steroidal estrogens and environmental protection.关键词
E2/假单胞菌/高效降解/响应面法/烟草废水处理Key words
E2/Pseudomonas sp.CO3/Efficientbiodegradation/RSM/Tobacco Wastewater Treatment分类
资源环境引用本文复制引用
孙福艳,廖成全,叶建斌,刘洪波,杨尘,杨成,齐晓娜,曾祥,黄家启,谭雅文..雌二醇降解菌的筛选鉴定及降解特性研究[J].云南化工,2024,51(8):56-61,6.基金项目
自然科学基金项目(4180071401). （4180071401）
