湖北医药学院学报2024，Vol.43Issue(4)：343-348,6.DOI:10.13819/j.issn.2096-708X.2024.04.002
蜂毒肽Protopolybia-MP Ⅲ及其优化突变体对HSV-1病毒复制周期不同阶段的抑制活性研究
Inhibitory Activity of Wasp Venom Peptide Protopolybia-MP Ⅲ and Its Optimized Mutants on HSV-1 Replica-tion of Different Stages
摘要
Abstract
Objective To study the inhibitory activity of the wasp venom peptide Protopolybia-MP Ⅲand its optimized mu-tants MP Ⅲ-3/4/7/10/14 on different stages of herpes simplex virus-1(HSV-1)replication in vitro.Methods The pre-ventive effects of Protopolybia-MP Ⅲ and its five optimized mutants on HSV-1 infection in Vero cells,as well as their effects on different stages of HSV-1 replication,were detected using qPCR and a peptide incubation platform.Results The MP Ⅲ-4/10/14 limited HSV-1 infection after the removal of the supernatant in preincubated Vero cells.Further analysis of the stages of antiviral activity of the peptides showed that the wild-type peptide Protopolybia-MP Ⅲmainly acted on the attachment of HSV-1 replication;MP Ⅲ-3/14 mainly on the entry/fusion and post entry of the virus replication cycle;MP Ⅲ-4/7 mainly acted on the attachment and entry/fusion stages;MP Ⅲ-10 mainly on the attachment and entry stages.Conclusion This paper determined the inhibitory activity of Protopolybia-MP Ⅲand its mutants MP Ⅲ-3/4/7/10/14 on different stages of HSV-1 replication and preliminarily clarified the reasons for the improved antiviral activity of optimized mutants of Protopolybia-MP Ⅲ.This study laid the foundation for the molecular design and medicinal research of wasp an-tiviral peptides.关键词
胡蜂毒液多肽/优化突变体/Ⅰ型单纯疱疹病毒/复制周期Key words
Wasp venom peptide/Optimized mutants/Herpes simplex virus-1/Replication cycle引用本文复制引用
孙芳,邬理莉,覃陈虎,罗旭东,陈宗运,叶祥东..蜂毒肽Protopolybia-MP Ⅲ及其优化突变体对HSV-1病毒复制周期不同阶段的抑制活性研究[J].湖北医药学院学报,2024,43(4):343-348,6.基金项目
国家自然科学基金青年项目(82204407) （82204407）
湖北省科技厅自然科学基金项目(2023AFB296) （2023AFB296）
湖北医药学院青年学者培养项目(2021QDJZR019) （2021QDJZR019）
