现代畜牧科技Issue(8)：1-7,7.DOI:10.19369/j.cnki.2095-9737.2024.08.001
25株猪肠道无毒力且不耐药大肠杆菌的分离鉴定、生长特性及遗传进化分析
Isolation,Identification,Growth Characteristics and Genetic Evolution of 25 Non-virulent and Non-antibiotic Resistant Escherichia coli Strains Isolated From Pig Intestines
摘要
Abstract
The aim of this study was to isolate Escherichia coli strains without virulence genes and drug resistance from pig intestines,and to analyze their growth performance and genetic evolution relationships.PCR was used to detect 13 virulence genes commonly found in porcine pathogenic E.coli,and susceptibility tests were conducted for 7 classes(14 types)of antibiotics.The growth curves of the non-virulent and non-resistant strains were determined,and a phylogenetic tree was constructed based on the 16S rRNA gene sequences.The results showed that 206 E.coli strains were isolated and identified in this study.Among them,107 E.coli strains did not contain 13 tested virulence genes,of which 25 strains were sensitive to 7 classes of antibiotics(14 kinds),and these 25 strains(non-virulent and non-resistant)showed similar growth performance to 3 typical E.coli strains.16S rRNA phylogenetic tree analysis showed that 4 E.coli strains were close to typical probiotic strains.Based on the results of growth curves and 16S rRNA phylogenetic tree,the four strains may be high-quality native Escherichia coli strains in pig gut and have research potential.关键词
大肠杆菌/毒力基因/抗生素/耐药性/生长/进化Key words
E.coli/virulence gene/antibiotic/drug resistance/growth/evolution分类
农业科技引用本文复制引用
区炳明,李清青,陈晓洁,萧碧扬,林雪,钟炜楠,张敏瑜..25株猪肠道无毒力且不耐药大肠杆菌的分离鉴定、生长特性及遗传进化分析[J].现代畜牧科技,2024,(8):1-7,7.基金项目
肇庆学院农村科技特派员工作站(2022N001) （2022N001）
肇庆学院大学生创新创业训练计划项目(X202310580123) （X202310580123）
