中国病理生理杂志2024，Vol.40Issue(8)：1361-1368,8.DOI:10.3969/j.issn.1000-4718.2024.08.001
超保守RNA uc.102-uc.106及其宿主基因OLA1在不同组织中的差异表达
Differential expression of ultraconserved RNA uc.102-uc.106 cluster and its host gene OLA1 in various tissues
摘要
Abstract
AIM:This study aims to investigate the expression differences of the ultraconserved RNA gene cluster uc.102-uc.106 and its host gene Obg-like ATPase 1(OLA1)in various tissues of C57BL/6J mice.METHODS:Five healthy male C57BL/6J mice aged 8～10 weeks were selected,and under normal physiological conditions,various tis-sues and organs,including liver,testis,bone marrow,brain,kidney,blood,lung,colon,thymus,spleen,stomach,heart,lymph nodes and bladder,were collected.Simultaneously,magnetic bead separation technology was employed to extract bone marrow blood cells,including B cells,macrophages,erythroid precursor,and mature red blood cells.The ex-pression levels of uc.102-uc.106 and OLA1 mRNA were determined by RT-qPCR with GAPDH as the internal reference.RESULTS:The OLA1 mRNA exhibited high expression levels in the brain,lymph nodes,testis and thymus of C57BL/6J mice,and low expression levels in the liver,spleen,lung,colon and peripheral blood(P<0.05).The expression trend of the uc.102-uc.106 gene cluster was generally opposite to that of OLA1 mRNA,except for both exhibiting high expression levels in testicular tissue(P<0.05).In blood cells,the OLA1 mRNA and the uc.102-uc.106 gene cluster showed the highest expression levels in B cells and the lowest expression levels in mature red blood cells(P<0.05).CONCLU-SION:This study indicates that OLA1 gene and uc.102-uc.106 gene cluster are expressed in different tissues and blood cells of C57BL/6J mice.Notably,the high expression levels of OLA1 gene and uc.102-uc.106 gene cluster in the mouse testis suggest a potential association with male reproductive functions.关键词
超保守RNA/uc.102-uc.106基因簇/OLA1基因/C57BL/6J小鼠Key words
ultraconserved RNA/uc.102-uc.106 gene cluster/OLA1 gene/C57BL/6J mice分类
生物科学引用本文复制引用
王鑫,张毅,许晗,魏子辰,庞磊,赵明超,郁多男..超保守RNA uc.102-uc.106及其宿主基因OLA1在不同组织中的差异表达[J].中国病理生理杂志,2024,40(8):1361-1368,8.基金项目
国家自然科学基金资助项目(No.81670186) （No.81670186）
