中国病理生理杂志2024，Vol.40Issue(8)：1369-1377,9.DOI:10.3969/j.issn.1000-4718.2024.08.002
ADAMDEC1通过Wnt/β-catenin信号通路调控胰腺癌细胞的生长和转移
ADAMDEC1 regulates growth and migration of pancreatic carcinoma cells through Wnt/β-catenin signaling pathway
摘要
Abstract
AIM:To investigate the effect of a disintegrin and metalloproteinase(ADAM)domain-like decy-sin 1(ADAMDEC1)knockdown on the proliferation,migration and invasion of pancreatic carcinoma cells.METHODS:Expression levels of ADAMDEC1 in pancreatic carcinoma tissues were analyzed using the GEPIA and UALCAN online da-tabases.Western blot analysis was employed to detect the protein expression levels of ADAMDEC1 in pancreatic carcino-ma cell lines(MIA PaCa-2 and PANC-1)and pancreatic ductal cell line(hTERT-HPNE).The effects of ADAMDEC1 knockdown on cell proliferation,migration and invasion were evaluated using CCK-8,colony formation,wound-healing and Transwell assays.Additionally,Western blot analysis was used to detect the effects of ADAMDEC1 knockdown on the expression levels of migration and invasion markers,as well as Wnt/β-catenin signaling pathway-related proteins in pancre-atic carcinoma cells.Furthermore,a recovery experiment was conducted to assess the role of Wnt/β-catenin signaling path-way agonist CHIR-99021 in ADAMDEC1 knockdown-induced inhibition of pancreatic carcinoma cell growth and migra-tion.RESULTS:(1)ADAMDEC1 was highly expressed in pancreatic carcinoma cells.(2)Knockdown of ADAMDEC1 led to a significant reduction in the proliferation,migration and invasion of pancreatic carcinoma cells.(3)Knockdown of ADAMDEC1 resulted in increased E-cadherin protein expression and decreased levels of matrix metalloproteinase 9,N-cadherin and vimentin proteins,alongside a reduction in the expression of Wnt/β-catenin signaling pathway-related pro-teins.(4)Co-treatment of pancreatic carcinoma cells with CHIR-99021 and ADAMDEC1 small interfering RNA reversed the inhibitory effects of ADAMDEC1 knockdown on cell proliferation,migration,and invasion.CONCLUSION:ADAMDEC1 is highly expressed in pancreatic carcinoma.Targeted silencing of ADAMDEC1 has the potential to inhibit the prolifera-tion,migration and invasion of pancreatic carcinoma cells by regulating the Wnt/β-catenin signaling pathway.关键词
胰腺癌/ADAMDEC1蛋白/细胞增殖/细胞迁移/细胞侵袭/Wnt/β-catenin信号通路Key words
pancreatic carcinoma/ADAMDEC1 protein/cell proliferation/cell migration/cell invasion/Wnt/β-catenin signaling pathway分类
医药卫生引用本文复制引用
黄小勇,樊心悦,徐向荣,蔺晓银,刘雨思,史海燕,杜娟,景红梅..ADAMDEC1通过Wnt/β-catenin信号通路调控胰腺癌细胞的生长和转移[J].中国病理生理杂志,2024,40(8):1369-1377,9.基金项目
延安市重点研发计划项目(No.2022SLSFGG-051) （No.2022SLSFGG-051）
