中国病理生理杂志2024，Vol.40Issue(8)：1384-1391,8.DOI:10.3969/j.issn.1000-4718.2024.08.004
维生素D受体在肺腺癌高表达及其对肺腺癌细胞活力和转移能力影响的研究
High expression of vitamin D receptor in lung adenocarcinoma and its in-fluence on viability and metastasis ability of lung adenocarcinoma cells
摘要
Abstract
AIM:To explore the expression level of vitamin D receptor(VDR)in lung adenocarcinoma and its impact on the biological function of lung adenocarcinoma cells.METHODS:The mRNA expression level of VDR in lung adenocarcinoma and its relationship with prognosis were analyzed through 6 lung adenocarcinoma datasets(compris-ing a total of 792 lung adenocarcinoma tissues and 230 adjacent non-tumor tissues).Immunohistochemistry was used to de-tect VDR protein expression in 30 lung cancer patients.Lung adenocarcinoma cell lines A549 and H1650 were studied,with transfection of negative control(NC)or two VDR short hairpin RNAs(VDR-shRNAs).CCK-8 assay compared the cell viability of cells in each experimental group.Transwell and wound-healing assays compared the invasion and migra-tion capabilities.Gene set enrichment analysis identified pathways enriched in lung adenocarcinoma tissues with high VDR expression.RESULTS:The mRNA expression level of VDR was significantly increased in lung adenocarcinoma tissues(P<0.01).Immunohistochemistry further confirmed the high expression of VDR in lung adenocarcinoma(P<0.01).The survival analysis showed that the expression of VDR had no significant effect on the overall survival of lung ad-enocarcinoma patients(P>0.05).Knockdown of VDR significantly inhibited the cell viability,invasion,and migration ca-pacity of lung adenocarcinoma cells(P<0.05).Gene set enrichment analysis showed that lung adenocarcinoma tissues with high VDR expression were enriched in signaling pathways such as epithelial-mesenchymal transition(P<0.05).CONCLUSION:VDR is highly expressed in lung adenocarcinoma,and VDR knockdown can inhibit the cell viability,in-vasion,and migration capacity of lung adenocarcinoma cells.关键词
维生素D受体/肺腺癌/细胞活力/细胞侵袭/细胞迁移Key words
vitamin D receptor/lung adenocarcinoma/cell viability/cell invasion/cell migration分类
医药卫生引用本文复制引用
邹淑梅,包振明,叶嘉,余宗阳,宋颖芳..维生素D受体在肺腺癌高表达及其对肺腺癌细胞活力和转移能力影响的研究[J].中国病理生理杂志,2024,40(8):1384-1391,8.基金项目
福建省自然科学基金面上项目(No.2020J011137) （No.2020J011137）
联勤保障部队第九〇〇医院临床医学研究专项(No.2020L13) （No.2020L13）
