中国病理生理杂志2024，Vol.40Issue(8)：1392-1398,7.DOI:10.3969/j.issn.1000-4718.2024.08.005
七甲氧基黄酮抑制人结直肠癌细胞增殖、迁移和侵袭的机制研究
Effect of heptamethoxyflavone on proliferation,migration and invasion of human colorectal cancer cells and its mechanism
摘要
Abstract
AIM:This study is aimed to investigate the impact of 3,5,6,7,8,3',4'-heptamethoxyflavone(HMF)on the proliferation,invasion,and migration of human colorectal cancer(CRC)cell lines(SW480 and HCT116)and preliminarily explore the underlying molecular mechanisms.METHODS:Human colorectal cancer cells(SW480 and HCT116)cultured in vitro were subjected to various concentrations of HMF(0,12.5,25 and 50 µmol/L)for 48 h.Proliferation levels were assessed using the CCK-8 assay,invasion abilities were examined via the Transwell assay,migra-tion rates were measured using the scratch assay,and oxidative stress levels were determined by the DCF-DA reactive oxy-genation assay.The mRNA expression levels of heme oxygenase-1(HO-1)mRNA and NAD(P)H:quinone oxidoreduc-tase-1(NQO-1)were quantified using RT-qPCR.RESULTS:Treatment with varying concentrations of HMF resulted in a significant reduction in the proliferative capacity of SW480 and HCT116 cancer cells,as was indicated by CCK-8 experi-ments(P<0.05).Transwell assays demonstrated a pronounced attenuation in the invasive potential of SW480 and HCT116 following HMF treatment(P<0.05).Scratch assays highlighted a notable constraint on the migratory capabilities of SW480 and HCT116 after HMF treatment(P<0.05).DCF-DA staining revealed a substantial increase in reactive oxy-gen species(ROS)levels within SW480 and HCT116 cells after HMF treatment(P<0.05).Furthermore,RT-qPCR ex-periments elucidated that HMF markedly suppressed the mRNA expression of antioxidant genes HO-1 and NQO-1.CON-CLUSION:HMF induces oxidative stress response in SW480 and HCT116 cells,consequently inhibiting their prolifera-tion,invasion and migration.关键词
七甲氧基黄酮/结直肠癌/细胞增殖/细胞迁移/细胞侵袭/氧化应激Key words
heptamethoxyflavone/colorectal cancer/cell proliferation/cell migration/cell invasion/oxida-tive stress分类
医药卫生引用本文复制引用
许诗琪,陈映彤,庄曼,余耿鑫,王晓燕,蔡轶,顾少菊..七甲氧基黄酮抑制人结直肠癌细胞增殖、迁移和侵袭的机制研究[J].中国病理生理杂志,2024,40(8):1392-1398,7.基金项目
广东省医学科学技术研究基金项目(No.A2022063) （No.A2022063）
广州医科大学大学生科技创新项目(No.2022A095) （No.2022A095）
