中国病理生理杂志2024，Vol.40Issue(8)：1399-1407,9.DOI:10.3969/j.issn.1000-4718.2024.08.006
特异性lncSLC25a6在同型半胱氨酸诱导的大鼠心肌细胞铜死亡中的作用
Role of specific lncSLC25a6 in homocysteine-induced cuproptosis in rat cardiomyocytes
摘要
Abstract
AIM:To investigate the role of specific long noncoding RNA SLC25a6(lncSLC25a6)in homocys-teine(Hcy)-induced cuproptosis in cardiomyocytes.METHODS:Rat cardiomyocytes were cultured in vitro and divided into control group and Hcy group.After 48 h of intervention,the expression levels of cuproptosis-related proteins,ferre-doxin 1(FDX1)and heat shock protein 70(HSP70),were detected by Western blot and immunofluorescence staining.The oxidative stress state of cardiomyocytes was assessed using fluorescence staining,and the intracellular Cu2+levels were measured using a copper ion assay kit.Furthermore,the impact of Hcy on the expression of cuproptosis-related proteins in cardiomyocytes was analyzed following overexpression of lncSLC25a6.RESULTS:Compared with the control group,80 µmol/L Hcy significantly accelerated cardiomyocyte damage,with a notable underexpression of lncSLC25a6(P<0.05).Western blot results indicated that,compared with the control group,the expression level of FDX1 in the Hcy intervention group was significantly reduced(P<0.05),while the expression level of HSP70 was significantly elevated(P<0.05),and the expression level of copper ions in cardiomyocytes of the Hcy group was increased(P<0.05).Immunofluorescence staining showed a significant reduction in FDX1 fluorescence intensity and a significant increase in HSP70 fluorescence in-tensity in the Hcy group.Further overexpression of lncSLC25a6 significantly mitigated Hcy-induced cuproptosis in cardio-myocytes,resulting in elevated expression of FDX1 and reduced expression of HSP70(P<0.05).Pearson correlation analysis demonstrated that the expression level of lncSLC25a6 was negatively correlated with FDX1 protein expression(r=-0.676,P=0.046)and positively correlated with HSP70 expression(r=0.680,P=0.044).CONCLUSION:lnc-SLC25a6 significantly mitigates Hcy-induced cuproptosis in cardiomyocytes,positioning it as a potential therapeutic target for managing Hcy-induced cardiac injury.关键词
lncSLC25a6/同型半胱氨酸/心肌细胞/铜死亡Key words
lncSLC25a6/homocysteine/cardiomyocytes/cuproptosis分类
医药卫生引用本文复制引用
李淑娟,姜怡邓,杨力,马胜超,黄晖,迟宏扬,汪乐新,何天宇,马福军,田彦成,赵彩齐,彭红建..特异性lncSLC25a6在同型半胱氨酸诱导的大鼠心肌细胞铜死亡中的作用[J].中国病理生理杂志,2024,40(8):1399-1407,9.基金项目
国家自然科学基金青年项目(No.81900273) （No.81900273）
国家自然科学基金面上项目(No.82060139 （）
No.82270492) （）
宁夏回族自治区重点研发计划项目(No.2019BEG03006) （No.2019BEG03006）
宁夏自然科学基金优秀青年项目(No.2023AAC05035) （No.2023AAC05035）
宁夏医科大学校级重点项目(No.XZ2022004) （No.XZ2022004）
