中国病理生理杂志2024，Vol.40Issue(8)：1426-1435,10.DOI:10.3969/j.issn.1000-4718.2024.08.009
姜黄素通过抑制JNK介导的炎症缓解慢性束缚应激诱导的大鼠心脏功能障碍
Curcumin alleviates chronic restraint stress-induced cardiac dysfunction in rats by inhibiting JNK-mediated inflammation
摘要
Abstract
AIM:To investigate the effects of curcumin on cardiac dysfunction induced by chronic restraint stress in a depression rat model.METHODS:Thirty-two Wistar rats weighing(200±20)g were randomly divided into control,model,low-dose curcumin,and high-dose curcumin groups(n=8 per group).The rats in model and curcumin groups were subjected to chronic restraint stress for 5 h daily at random time,while those in control group were maintained under normal conditions.Following daily stress exposure,the rats in low-and high-dose curcumin groups received 100 and 200 mg/kg curcumin daily,respectively,and those in control and model groups received the same volume of normal saline daily.The above treatments lasted for 28 d.Body weight of the rats was measured weekly.Sucrose preference test was performed on days 14 and 28 of the experiment.Serum corticosterone content was determined to evaluate depression.Histological changes of cardiac tissues were observed using HE and Masson staining.Echocardiography was conducted to examine heart function.The related mRNA and protein levels were detected using RT-qPCR and Western blot,respective-ly.RESULTS:Compared with control group,the rats in model group exhibited significantly slower weight gain(P<0.05),impaired sucrose preference(P<0.01),and increased corticosterone levels(P<0.01).HE staining revealed myo-cardial hypertrophy in model group but not in control group.Masson staining indicated significantly higher cardiac fibrosis in model group than control group(P<0.01).Immunohistochemical staining demonstrated a significant increase in posi-tive collagen type I expression(P<0.01).RT-qPCR results showed significantly elevated mRNA levels of inflammatory cytokines(tumor necrosis factor-α,interleukin-6,and interleukin-1β)and fibrosis factors(α-smooth muscle actin,colla-gen type I,and collagen type Ⅲ)in model group compared with control group(P<0.05 or P<0.01).Western blot re-vealed a significant increase in c-Jun N-terminal kinase(JNK)phosphorylation level in model group(P<0.01).Treat-ment with low-and high-dose curcumin reversed the above indicators.CONCLUSION:Curcumin treatment attenuated cardiac inflammation and fibrosis in rats subjected to chronic restraint stress,possibly by inhibiting JNK signaling pathway.关键词
姜黄素/慢性束缚应激/抑郁症/心血管疾病/炎症/纤维化/JNK信号通路Key words
curcumin/chronic restraint stress/depression/cardiovascular diseases/inflammation/fibrosis/JNK signaling pathway分类
医药卫生引用本文复制引用
姚倩,朱家峰,杨茂全,徐悦,秦丽丽,吴升芹,张坤英..姜黄素通过抑制JNK介导的炎症缓解慢性束缚应激诱导的大鼠心脏功能障碍[J].中国病理生理杂志,2024,40(8):1426-1435,10.基金项目
潍坊市卫生健康委员会项目(No.WFWSJK-2020-200) （No.WFWSJK-2020-200）
