中国当代儿科杂志2024，Vol.26Issue(9)：967-973,7.DOI:10.7499/j.issn.1008-8830.2405084
川芎嗪通过激活SIRT1信号通路减轻内皮细胞炎症损伤的机制研究
Mechanism of tetramethylpyrazine attenuates inflammatory injury in endothelial cells by activating the SIRT1 signaling pathway
摘要
Abstract
Objective To study the effects and mechanisms of tetramethylpyrazine(TMP)on tumor necrosis factor-α(TNF-α)-induced inflammatory injury in human coronary artery endothelial cells(HCAEC).Methods HCAEC were randomly divided into four groups:the control group(no treatment),the model group(treated with TNF-α,50 ng/mL for 24 hours),the TMP group(pre-treated with TMP,80 μg/mL for 12 hours followed by TNF-α treatment for 24 hours),and the SIRT1 inhibitor group(pre-treated with TMP and the specific SIRT1 inhibitor EX527 for 12 hours followed by TNF-α treatment for 24 hours).Cell viability was assessed using the CCK-8 method,lactate dehydrogenase(LDH)activity was measured using an LDH assay kit,reactive oxygen species(ROS)levels were observed using DCFH-DA staining,expression of pyroptosis-related proteins was detected by Western blot,and SIRT1 expression was analyzed using immunofluorescence staining.Results Compared to the control group,the model group showed decreased cell viability,increased LDH activity,ROS level and expression of pyroptosis-related proteins,and decreased SIRT1 expression(P<0.05).Compared to the model group,the TMP group exhibited increased cell viability,decreased LDH activity,ROS level and expression of pyroptosis-related proteins,and increased SIRT1 expression(P<0.05).In comparison to the TMP group,the SIRT1 inhibitor group showed decreased cell viability,increased LDH activity,ROS level and expression of pyroptosis-related proteins,and decreased SIRT1 expression(P<0.05).Conclusions TMP may attenuate TNF-α-induced inflammatory injury in HCAEC,which is associated with the inhibition of pyroptosis and activation of the SIRT1 signaling pathway.关键词
炎症损伤/川芎嗪/SIRT1/焦亡/人冠状动脉内皮细胞Key words
Inflammatory injury/Tetramethylpyrazine/SIRT1/Pyroptosis/Human coronary artery endothelial cell引用本文复制引用
陈露萍,杨轶童,赵苗苗,李翰文,孙文婷,石曌玲..川芎嗪通过激活SIRT1信号通路减轻内皮细胞炎症损伤的机制研究[J].中国当代儿科杂志,2024,26(9):967-973,7.基金项目
国家自然科学基金青年科学基金项目(82205188) （82205188）
陕西省重点研发计划项目(2023-YBSF-638). （2023-YBSF-638）
