中国防痨杂志2024，Vol.46Issue(9)：1056-1062,7.DOI:10.19982/j.issn.1000-6621.20240167
17种抗生素对北京地区快速生长分枝杆菌体外抑菌效果分析
Analysis of in vitro antibacterial effects of 17 antibiotics against rapidly growing mycobacteria in the Beijing area
摘要
Abstract
Objective:To analyze the antibacterial effects of 17 antibiotics on clinical isolates of rapidly growing mycobacteria(RGM)in Beijing,and to provide a reliable theoretical basis for the clinical treatment of nontuberculosis mycobacteriosis caused by RGM.Methods:A total of 130 nontuberculous mycobacteria(NTM)strains isolated from the Tuberculosis Laboratory of the Beijing Center for Disease Control and Prevention between January 2016 and December 2020 were collected for strain identification.After reviving the RGM clinical isolates and corresponding standard strains,sensitivity tests were conducted on 17 antibiotics(amikacin,ciprofloxacin,compound sulfamethoxazole,clarithromycin,linezolid,moxifloxacin,doxycycline,minocycline,cefoxitin,tigecycline,tobramycin,imipenem,meropenem,amoxicillin/clavulanic acid,bedaquiline,delamanid,and clofazimine)using the micro broth dilution method.The minimum inhibitory concentration(MIC)and the MIC values required to inhibit 50％(MIC50)or 90％(MIC90)of the tested bacteria were recorded.Results:After strain identification and successful recovery,37 strains of RGM were identified,including 19 Mycobacterium(M.)abscesses,17 M.fortuitum,and 1 M.chelonae.Among the 14 antibiotics with established resistance breakpoints,all RGM clinical isolates were found to be sensitive to tigecycline.Clinical isolates of M.chelonae and M.abscessus were sensitive to clarithromycin,and M.abscesses were also sensitive to linezolid.All M.fortuitum clinical isolates were sensitive to amikacin,ciprofloxacin,compound sulfamethoxazole,and moxifloxacin.Additionally,the sensitivity rate of M.abscessus to amikacin was 73.7％(14/19),while the sensitivity rates of M.fortuitum to linezolid and meropenem were 82.4％(14/17)and 88.2％(15/17),respectively.All M.abscessus isolates were resistant to doxycycline,amoxicillin/clavulanic acid,while all M.fortuitum isolates were resistant to tobramycin.Additionally,the MIC9o of bedaquiline and clofazimine for RGM clinical isolates were both lower than the minimum detection limit of the drug sensitivity plate,whereas the MIC90 of delamanid was higher than the maximum detection limit of the drug sensitivity plate.Conclusion:Tigecycline,amikacin,linezolid,betaquiline,and clofazimine all exhibit good in vitro antibacterial activity against RGM,while doxycycline,amoxicillin/clavulanic acid,and delamanid show almost no in vitro antibacterial activity.There is a significant difference in the drug resistance spectrum between NTM species.Therefore,it is recommended to conduct species identification and drug sensitivity testing before treatment to select appropriate antibiotics.关键词
分枝杆菌,非典型性/快速生长分枝杆菌/微生物敏感性试验/评价研究Key words
Mycobacteria,atypical/Rapidly growing mycobacteria/Microbial sensitivity tests/Evaluation studies分类
医药卫生引用本文复制引用
陈双双,田丽丽,王嫩寒,杨新宇,赵琰枫,李传友,代小伟..17种抗生素对北京地区快速生长分枝杆菌体外抑菌效果分析[J].中国防痨杂志,2024,46(9):1056-1062,7.基金项目
Self Managed Project of Tuberculosis Control Institute in Beijing(JKS2021-02) 北京结核病控制研究所自管课题(JKS2021-02) （JKS2021-02）
