中国临床药理学杂志2024，Vol.40Issue(16)：2339-2343,5.DOI:10.13699/j.cnki.1001-6821.2024.16.008
高碘通过内质网应激和P38MAPK信号通路诱导甲状腺上皮细胞损伤的研究
Research of high iodine induced damage to thyroid epithelial cells through endoplasmic reticulum stress and P38MAPK signaling pathway
摘要
Abstract
Objective To investigate the role of endoplasmic reticulum stress and p38 mitogen-activated protein kinase P38MAPK signaling pathway in thyroid epithelial cell injury induced by high iodine.Methods The thyroid epithelial cells Nthy-ori 3-1 were randomly divided into control group(normal culture),model group(40 mmol·L-1 potassium iodide),4-phenylbutyric acid(4-PB A)group(40 mmol·L-1 potassium iodide and 2 mmol·L-1 4-PBA)and SB203580 group(40 mmol·L-1 potassium iodide and 10 μmol·L-1 SB203580).Western blot was used to detect the expression of glucose regulated protein 78(GRP78)and p-P38/P38 of Nthy-ori 3-1 cells.MTT and colony formation experiments were used to detect the proliferation level.Flow cytometry was used to detect the apoptosis level.Enzyme-linked immunosorbent assay(ELISA)was used to detect the level of interleukin-6(IL-6).Results The expression levels of GRP78 protein in control group,model group,4-PBA group and SB203580 group were 0.15±0.03,0.61±0.07,0.27±0.03 and 0.37±0.04;the ratios of p-P38/P38 were 0.12±0.03,0.53±0.04,0.35±0.04 and 0.25±0.03;cell survival rates were(100.00±0.00)％,(53.71±6.16)％,(80.24±8.17)％and(71.29±7.36)％;the number of clones formed was 271.36±25.18,96.09±10.79,183.24±15.36 and 141.24±16.18;the apoptosis rates were(1.04±0.21)％,(9.27±1.67)％,(3.18±1.52)％and(3.82±1.09)％;IL-6 secretion levels were(0.71±0.08),(9.17±0.87),(3.26±0.29)and(4.71±0.41)nmol·L-1,respectively.For the above indicators,there was significant difference between the model group and the control group(all P＜0.05);there was significant difference between the 4-PBA group,SB203580 group and the model group(all P＜0.05).Conclusion High iodine can inhibit the proliferation of Nthy-ori 3-1 cells and induce apoptosis and secretion of inflammatory factors,which may be related to the activation of endoplasmic reticulum stress and P38MAPK signaling pathway by high iodine.关键词
高碘/内质网应激/p38丝裂原活化蛋白激酶/甲状腺上皮细胞/凋亡Key words
high iodine/endoplasmic reticulum stress/p38 Mitogen-Activated Protein Kinase/thyroid epithelial cell/apoptosis分类
医药卫生引用本文复制引用
杨鑫娜,蔡辉耀,陈丽君,梁波..高碘通过内质网应激和P38MAPK信号通路诱导甲状腺上皮细胞损伤的研究[J].中国临床药理学杂志,2024,40(16):2339-2343,5.基金项目
福建省中青年教师教育科研项目(科技类)立项基金资助项目(JAT200163) （科技类）
