中国临床药理学杂志2024，Vol.40Issue(16)：2413-2417,5.DOI:10.13699/j.cnki.1001-6821.2024.16.024
射干麻黄汤与定喘汤治疗支气管哮喘寒热证候的药理学分析
Pharmacological of Shegan Mahuang decoction and Dingchuan decoction in the treatment of cold and heat syndrome of bronchial asthma
摘要
Abstract
Objective To analyze the difference in mechanism between Shegan Mahuang decoction and Dingchuan decoction in treating cold and heat syndrome of bronchial asthma.Methods Use the traditional Chinese medicine system pharmacology database and analysis platform to screen the effective ingredients and targets of each component of Shegan Mahuang decoction and Dingchuan decoction,respectively.Obtain gene targets corresponding to bronchial asthma through gene disease association databases and human gene information databases,screen the core targets and components of Shegan Mahuang decoction and Cold Asthma,Dingchuan decoction and Heat Asthma,and perform network topology analysis using online network analysis platforms.Perform gene ontology(GO)and Kyoto Encyclopedia of Genomes(KEGG)functional enrichment analysis using the"org.Hs.eg.db"software package in R software,predict their different mechanisms of action,and use Autodock Vina software to analyze and verify the molecular docking technology of core active ingredients and core targets.Results There are 130 active ingredients in Shegan Mahuang decoction,236 active ingredients in Dingchuan decoction,and 54 identical active ingredients.Among the targets related to bronchial asthma,Shegan Mahuang decoction has 52 potential therapeutic targets,and Dingchuan decoction has 45 potential therapeutic targets.GO and KEGG analysis showed that there were differences between Shegan Mahuang decoction and Dingchuan decoction in platelet activation,adipocyte cytokine signaling pathways,alcoholic liver disease,inflammatory mediator regulation of transient receptor potential channels,inflammatory bowel disease,adrenergic signaling in myocardial cells,phosphoinositide-3-kinase-protein kinase B signaling pathways,etc.There are differences in core compounds such as iristectorigenin(9CI)and iristectorigenin A in Shegan Mahuang decoction compared to core compounds such as arachidonic acid and glycyrrhetinic acid in Dingchuan decoction;the mechanism of action of Dingchuan decoction in treating heat asthma and Shegan Mahuang decoction in treating cold asthma differs in the targets of signal transduction and transcription activation factor 3(STAT3),tansient receptor potential vanilloid 1(TRPV1),and Ras homologous gene(RHO).Conclusion Two traditional Chinese medicine formulas regulate the different molecular and biological processes caused by asthma cold and heat syndromes,reflecting the biological differences between the two traditional Chinese medicine syndromes of cold asthma and heat asthma.关键词
射干麻黄汤/定喘汤/冷哮/热哮/网络药理学/分子对接技术Key words
Shegan Mahuang decoction/Dingchuan decoction/cold asthma/heat asthma/network pharmacology/molecular docking technique分类
医药卫生引用本文复制引用
陈丽平,韩立,卞华,庞立业..射干麻黄汤与定喘汤治疗支气管哮喘寒热证候的药理学分析[J].中国临床药理学杂志,2024,40(16):2413-2417,5.基金项目
国家自然科学基金青年科学基金资助项目(81704200) （81704200）
河南省自然科学基金资助项目(232300421192) （232300421192）
河南省科技攻关基金资助项目(222102310551) （222102310551）
第五批全国中医临床优秀人才研修基金资助项目(国中医药人教函[2022]239号) （国中医药人教函[2022]239号）
