中国免疫学杂志2024，Vol.40Issue(8)：1579-1583,1589,6.DOI:10.3969/j.issn.1000-484X.2024.08.002
基于酵母β-葡聚糖载体的口服OVA疫苗(WGP-OVA疫苗)诱导体液免疫及抗原特异性T细胞免疫反应
Yeast β-glucan vehicle based oral OVA vaccine(WGP-OVA vaccine)induces humoral and antigen-specific T cell immune responses
摘要
Abstract
Objective:To investigate humoral and antigen-specific T cell immune responses induced by oral administration of WGP-OVA in C57BL/6 mice.Methods:C57BL/6 mice were orally administered with PBS,WGP and WGP-OVA for 17 days,respec-tively.The serum immunoglobulin levels including IgG1,IgG2a,IgG3 and IgM of mice were detected using LEGENDplexTM Multi-An-alyte Flow Assay Kit.HE staining was used to compare the size of lymphoid nodules in spleens.FACS was used to analyze the percent-age of F4/80+macrophages and F4/80+SIINFEKL+macrophages in inguinal lymph nodes(ILNs).In addition,the T cell-mediated im-munity stimulated by the WGP-OVA oral vaccine was analyzed by ILNs-infiltrating antigen-specific CD8+T cell using MHC Tetramer assay.Results:Oral administration of WGP-OVA significantly increased the contents of IgG1,IgG2a,IgG3 and IgM in the serum,and enlarged the lymphoid nodules in spleens,compared to those orally administered with PBS.Oral administration of WGP-OVA pro-moted the ILNs-infiltration of F4/80+macrophages,and enhanced the antigen-presentation of OVA in macrophages in ILNs.Moreover,the expansion of OVA specific CD8+CTLs in ILNs was also stimulated by WGP-OVA.Conclusion:WGP-OVA involved in both the humoral and antigen-specific T cell immune responses in C57BL/6 mice.关键词
全β-葡聚糖颗粒/鸡卵清蛋白/口服疫苗/免疫球蛋白/抗原特异性T细胞Key words
Whole β-glucan particles/Ovalbumin/Oral vaccine/Immunoglobulins/Antigen-specific T cells分类
医药卫生引用本文复制引用
白煜,戚春建,夏蕾,何树燕,何流漾..基于酵母β-葡聚糖载体的口服OVA疫苗(WGP-OVA疫苗)诱导体液免疫及抗原特异性T细胞免疫反应[J].中国免疫学杂志,2024,40(8):1579-1583,1589,6.基金项目
国家自然科学基金(32270954) （32270954）
常州市科技计划项目(CJ20220082) （CJ20220082）
江阴市卫健委面上项目(S202101). （S202101）
