中国免疫学杂志2024，Vol.40Issue(8)：1601-1606,6.DOI:10.3969/j.issn.1000-484X.2024.08.006
槲皮素通过HMGB1/RAGE/NF-κB通路减轻癫痫大鼠神经炎症的实验研究
An experimental study of quercetin attenuating neuroinflammation in epileptic rats through HMGB1/RAGE/NF-κB pathway
摘要
Abstract
Objective:To investigate whether quercetin reduces neuroinflammation in epileptic rats by regulating high mobility group protein B1(HMGB1),receptor for advanced glycation end products(RAGE)and nuclear factor kappa-B(NF-κB),and to explore the possibility of HMGB1/RAGE/NF-κB pathway as a new target of quercetin.Methods:Twelve SD rats were randomly selected from 60 SD rats as healthy group,and the remaining rats were used to construct experimental models of epilepsy rats.Rats that were successfully modeled were divided into model group,high-dose quercetin group,low-dose quercetin group,quercetin+pathway activator group,with 12 rats in each group.Pathological changes of hippocampal tissue of rats were observed;neurobehavioral function of rats was evaluated;levels of tumor necrosis factor-α(TNF-α),IL-6 and IL-1β,and mRNA and protein expression levels of HMGB1,RAGE,NF-κB in hippocampal tissue of rats were detected.Results:Structure of hippocampus of rats in healthy group was complete;compared with healthy group,structure of hippocampal tissue of rats in model group was scattered,the number of surviving neurons was observably reduced,apoptotic index was observably increased,the Racine grade was observably increased,TNF-α,IL-1β,IL-6 contents and mRNA and protein expression levels of HMGB1,RAGE,NF-κB in hippocampal tissue were significantly increased;com-pared with model group,structure of hippocampal tissue of rats in low-and high-dose quercetin groups was relatively complete,the number of surviving neurons was observably increased,apoptotic index was observably decreased,the Racine grade was significantly decreased,TNF-α,IL-1β,IL-6 contents and mRNA and protein expression levels of HMGB1,RAGE,NF-κB were significantly re-duced,and the improvement effect of high-dose quercetin group was better;compared with high-dose quercetin group,the number of surviving neurons in quercetin+pathway activator group was significantly reduced,apoptotic index was significantly increased,the Ra-cine grade was significantly increased,TNF-α,IL-1β,IL-6 contents and mRNA and protein expression levels of HMGB1,RAGE,NF-κB in hippocampal tissue were significantly increased.Conclusion:Quercetin can effectively reduce neuroinflammation in epilep-tic rats by inhibiting the HMGB1/RAGE/NF-κB pathway and reducing the mRNA and protein expressions of related genes.关键词
槲皮素/癫痫/高迁移率族蛋白B1/糖基化终末产物受体/核转录因子κBKey words
Quercetin/Epilepsy/HMGB1/RAGE/NF-κB分类
医药卫生引用本文复制引用
吴琼莹,高文勇,艾艳萍,魏海棠,魏芬飞..槲皮素通过HMGB1/RAGE/NF-κB通路减轻癫痫大鼠神经炎症的实验研究[J].中国免疫学杂志,2024,40(8):1601-1606,6.基金项目
湖北省卫生健康委员会项目(WJ2017F042). （WJ2017F042）
