中国免疫学杂志2024，Vol.40Issue(8)：1726-1734,9.DOI:10.3969/j.issn.1000-484X.2024.08.024
ANLN在肾透明细胞癌中的表达及其与预后和免疫浸润相关性的生物信息学分析
Bioinformatics analysis of ANLN expression in clear cell renal cell carcinoma and its correlation with prognosis and immune infiltration
摘要
Abstract
Objective:To investigate diagnostic and prognostic value of actin-binding protein ANLN in clear cell renal cell carcinoma(ccRCC)and its relationship with tumor microenvironment.Methods:Gene expression data and clinical data for ccRCC were downloaded from The Cancer Genome Atlas(TCGA).Relationship between ANLN expression and clinicopathological features was assessed by Wilcoxon rank sum test and Logistic regression.Receiver operating characteristic(ROC)curve was used to assess diagnostic value of ANLN expression in ccRCC.Kaplan-Meier and Cox regression analysis were used to investigate effect of ANLN expression on overall survival.Gene set enrichment analysis(GSEA)was used to identify signaling pathways associated with ANLN in ccRCC.Relationship between ANLN expression and immune infiltration was analyzed by ESTIMATE algorithm,tumor immune estima-tion resource(TIMER)and CIBERSORT algorithms.Relationship between ANLN and drug sensitivity was calculated using CellMiner database.Results:ANLN expression was significantly upregulated in ccRCC tissues.ANLN expression in ccRCC was correlated with clinicopathological features.ROC analysis showed that ANLN had a high diagnostic value in ccRCC.High ANLN expression was signifi-cantly associated with poor prognosis.Multivariate Cox regression analysis showed that high ANLN expression was an independent risk factor for overall survival in ccRCC patients.GSEA showed that ANLN was associated with multiple signaling pathways.In terms of immunity,ANLN was closely associated with tumor microenvironment,immune infiltration and immune checkpoint molecules in ccRCC.ANLN expression was negatively correlated with sensitivity of most antitumor drugs.Conclusion:ANLN is a potential diagnos-tic and prognostic biomarker and immunotherapeutic target for ccRCC.关键词
ANLN/肾透明细胞癌/预后/生物标志物/免疫浸润Key words
ANLN/Clear cell renal cell carcinoma/Prognosis/Biomarker/Immune infiltration分类
医药卫生引用本文复制引用
刘东洋,张楠,李海玲,王昱锜,苏俞菠,高玉敏..ANLN在肾透明细胞癌中的表达及其与预后和免疫浸润相关性的生物信息学分析[J].中国免疫学杂志,2024,40(8):1726-1734,9.基金项目
内蒙古自治区卫生健康科技计划(202201228). （202201228）
