中国麻业科学2024，Vol.46Issue(4)：213-221,228,10.
工业大麻CsIAA1基因克隆及表达分析
Cloning and Expression Analysis of CsIAA1 Gene in Industrial Hemp
摘要
Abstract
In this study,the CsIAA1gene was cloned from the industrial hemp Y7.The sequence characteristics of CsIAA1were analyzed by bioinformatics,and the tissue-specific expression pattern of CsIAA1was studied by RT-qPCR.RT-qPCR was used to analyze the expression level of industrial hemp Y7 at different stages of Male development,and the CsIAA1 gene in different varieties of hemp was cloned and its amino acid sequence difference was analyzed.The results showed that the full-length cD-NA sequence of CsIAA1gene was 621 bp,encoding 206 amino acids,containing AUX_IAA conserved domain,which was an unstable protein.The phylogenetic tree analysis showed that the industrial hemp CsIAA1was closely related to Parasponia andersonii and Trema orientale.Through transient overexpres-sion and confocal experiments in tobacco leaves,it was found that CsIAA1was localized in the nucleus.Through qRT-PCR analysis,it was found that within 12 h of IAA treatment,CsIAA1had the highest ex-pression level of auxin at 0.5 h,indicating that CsIAA1gene could respond to exogenous IAA.In the male of different developmental stages of industrial hemp,the expression level of CsIAA1in the early stage of male development was higher than that in the development stage and the full-bloom stage,and it showed a gradual downward trend.It was speculated that CsIAA1was involved in regulating the male de-velopment of industrial hemp.Its specific function remains to be further studied.In this experiment,the auxin-responsive gene CsIAA1was cloned from industrial cannabis and its function was analyzed,which laid a foundation for the subsequent research on the mechanism of gender development of industrial can-nabis.关键词
工业大麻/CsIAA1/氨基酸序列/保守结构域/表达量Key words
industrial hemp/CsIAA1/amino acid sequence/conserved domains/expression quantity分类
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杨晓娟,阳锡涛,苏雨娟,黄彤,刘婕,高泽昕,栾明宝,潘根,黄思齐..工业大麻CsIAA1基因克隆及表达分析[J].中国麻业科学,2024,46(4):213-221,228,10.基金项目
中央科研院所基本科研业务费(1610242021006) （1610242021006）
