农学学报2024，Vol.14Issue(8)：56-62,7.
牛至精油的提取工艺优化及成分分析
Extraction Process Optimization and Component Analysis of Oregano Essential Oil
摘要
Abstract
The study aims to optimize the extraction conditions of essential oil from Oregano by traditional steam distillation and simultaneous distillation-extraction.The extraction parameters were optimized by single factor and orthogonal experiments,and the essential oil was analyzed by GC/MS.The results showed that the optimal extraction process combination of steam distillation and simultaneous distillation-extraction was the basification of 1%,with extraction time for 1.5 h,solid-liquid ratio of 1:6.Under these conditions,the extraction rates of steam distillation and simultaneous distillation-extraction were 0.486 g/100 g and 0.608 g/100 g,respectively.There were 16 kinds of alkanes,6 kinds of alkenes,4 kinds of alcohols,3 kinds of phenols and 1 kind of ketones in the oil obtained by conventional steam distillation,the contents of which accounted for 74.366%,6.263%,1.245%,12.684%and 0.449%,respectively.20 kinds of alkanes,9 kinds of alkenes,5 kinds of alcohols,3 kinds of phenols and 1 kind of ketones were obtained by simultaneous distillation-extraction,which accounted for 60.127%,12.597%,2.795%,22.147%and 0.936%,respectively.At the same time,the types and contents of main bacteriostatic components of essential oils obtained by simultaneous distillation-extraction were significantly higher than those of conventional steam distillation.The contents of thymol,cymene,γ-terpinene,carvacrol and α-hydrocene in conventional steam distillation essential oils were 8.492%,3.286%,2.551%,2.143%and 0.206%,respectively.And those of simultaneous distillation-extraction were 13.925%,5.218%,4.509%,4.14%and 0.36%,respectively.This study can provide reference for the production and preparation of oregano essential oil.The optimum technology obtained in this study has the high yield of essential oil,which can provide reference for the production and preparation of oregano essential oil.关键词
牛至精油/工艺优化/成分分析/正交试验Key words
oregano essential oil/process optimization/composition analysis/orthogonal test分类
农业科技引用本文复制引用
王莹,刘晨曦,王新炎,何际芳,李豪辉,贾陆,刘伟,薛刚..牛至精油的提取工艺优化及成分分析[J].农学学报,2024,14(8):56-62,7.基金项目
南阳市协同创新重大专项(郑州大学南阳研究院)项目"牛至的综合利用关键技术及应用研究"(NRIZU2020CIP0008) （郑州大学南阳研究院）
南阳理工学院交叉科学研究项目"中药牛至渣发酵替抗饲料活性成分变化规律研究"(NGJC-2022-22) （NGJC-2022-22）
南阳市科技攻关项目"精油类添加剂'替抗'抗氧化效应的研究"(KJGG077). （KJGG077）
