软腐病菌胁迫下甘薯生理生化响应及转录组分析OA北大核心CSTPCD

In order to clarify the physiological mechanism of soft rot disease and improve the quality of sweet potato during storage,the physiological and biochemical responses and transcriptomics of"Xinxiang",the major fresh eating variety of sweet potato in Zhejiang,were analyzed before and after disease infection.The results showed that:1)After infection with soft rot disease,the activities of antioxidant enzymes and cell wall-degrading enzymes in sweet potato significantly increased,and the ultrastructure analysis showed severe damage to the cell structure.2)During the process of sweet potato soft rot disease infection,12 205 genes showed significant changes,among which,7 505 displayed upregulated expression and 4 700 displayed downregulated expression;In the GO category,different expression genes were enriched in organelles,membranes and other cellular components,metabolic processes,biological activities,response to corresponding stimuli and catalytic activities in molecular functions.KEGG annotation analysis revealed that DEGs were significantly enriched in phenylpropane metabolism,carbon metabolism,starch and sucrose metabolism,amino acid biosynthesis,citric acid cycle,hormone signaling transduction and biosynthesis of secondary metabolites.This study provided a theoretical reference for further research on the gene function related to sweet potato disease resistance and the elucidation of disease resistance mechanisms.