石斛兰杂交后代iPBS标记鉴定及遗传分析OA北大核心CSTPCD

In order to obtain progeny with excellent traits for subsequent breeding of new varieties and selection of high-quality parents,using iPBS and morphological double marker identification to investigate the genetic identification of the hybrid combination of Dendrobium Fancy Pink Stripe(male parent)and Den.parishii'Damrong Star'3lip(female parent).The combination was genetically identified.The results showed that:The 36 progeny were identified true hybrids with iPBS markers.At the genetic distance of 0.71,the parents and 36 progeny could be divided into three groups,and the progeny were clustered with the male parents first and then aggregated with the female parent and the coefficients of genetic similarity between the parents and the progeny were male parent(0.686 1)＞female parent(0.510 5).Morphological identification showed significant differences in various traits between the two parents.The mean values of pseudobulb height,pseudobulb diameter,leaf length and leaf width in the hybrids generation were 25.48 cm,1.14 cm,14.17 cm and 3.45 cm,respectively.The size of plant and leaf type of the hybrid was mainly between the two parents;The mean values of transverse diameter and longitudinal diameter of the flower were 4.96 cm,and 5.75 cm respectively.The proportions of the two superior to their parents were 16.67％and 2.78％,respectively.And the majority of the hybrid plants had a smaller flower type than that of their parents.Among the floral traits,four traits(upper calyx width,lateral calyx length and lateral petal length)showed some supra-parental dominance,and two traits,labellum length and pedicel number,showed strong meso-parental dominance,while the rest of the traits showed different degrees of decline;The hybrid was dominated by the white-purple line of the parents in terms of the genetic inheritance of the floral colours,and showed gradient variations in the floral colours on the basis of the chromatograms of the two parents.In summary,iPBS molecular markers can effectively improve the efficiency of identifying Den.hybrids,and the morphological markers assists to efficiently select the hybrid individuals with excellent traits,and it can effectively shorten the breeding process of Den.crossbreeding by using these two types of marker.