光质交替对LED红蓝光连续光照生菜生理伤害的削减作用机制OA北大核心CSTPCD
Physiological mechanisms of light quality alternation in alleviating the injury on lettuce caused by continuous red and blue light illumination
为探明光质交替对LED红蓝光连续光照诱导生菜生理伤害的削减作用机制,在环境可控的植物工厂中,采用水培方法和昼夜6次取样方法(明期6 h间隔取样3次,暗期2 h间隔取样3次)研究相同日光照累积量下LED红蓝光常规光照(NL,光强 400 μmol/(m2·s),光周期 18 h/6 h,R/B=4∶1)、连续光照(CL,光强 300μmol/(m2·s),光周期24 h/0h,R/B=4∶1)和红蓝光交替连续光照(CL-A,红光320 μmol/(m2·s)照射18 h,蓝光240 μmol/(m2·s)照射6 h)对水培生菜生长、产量和昼夜自由基清除率(DPPH)及碳水化合物、活性氧和丙二醛(MDA)含量的影响.结果表明:光照处理12 d后生菜叶片产生了生理伤害并改变了植株形态.CL-A处理地上部鲜重、叶面积最高,冠幅更大,叶片伤害等级最低.按NL、CL和CL-A顺序,生菜叶片淀粉和蔗糖含量、DPPH呈现显著递增趋势,过氧化氢和MDA含量呈现先增后降趋势,而超氧阴离子含量呈现增加或先增后降趋势.光质交替削减了连续光照诱导的生菜产生的生理伤害,该研究结果为光质交替连续光照在人工光植物工厂中的应用提供了科学支撑.
In environmentally controlled plant factory,the effects of conventional light(NL,400 μmol/(m2·s),18 h/6 h photoperiod,R/B=4∶1),continuous light(CL,300 μmol/(m2·s),24 h/0 h photoperiod,R/B=4∶1)and spectral alternation continuous light(CL-A,red light with 320 μmol/(m2·s)and 18 h light period,blue light 240 μmol/(m2·s)with6 h light period,respectively)on the growth,yield,DPPH radical scavenging rate,and contents of carbohydrate,reactive oxygen species and MDA of lettuce were investigated via hydroponic culture and diurnal six sampling method(3 times at 6 h interval in the light period and 3 times at 2 h interval in the dark period).The results showed that:The lettuce leaves were damaged and the plant morphology was changed after 12 days treatment.The shoot fresh weight and leaf area of lettuce were the highest and the injury grade was the lowest under CL-A treatment.In NL,CL and CL-A order,the contents of starch and sucrose,the DPPH free radical scavenging rate increased gradually significantly,while the contents of hydrogen peroxide and MDA increased first and then decreased,but the superoxide anions content increased first and then decreased or always increased.In conclusion,light quality alternation alleviates the injury on lettuce caused by continuous red and blue light illumination.This study provides scientific support for the application of alternating light quality and continuous lighting in artificial light plant factories.
刘文科;刘家源
中国农业科学院农业环境与可持续发展研究所/农业农村部设施农业节能与废弃物处理重点实验室,北京 100081
生菜连续光照光质交替碳水化合物活性氧
lettucecontinuous lightspectral alternationcarbohydratereactive oxygen species
《中国农业大学学报》 2024 (010)
131-138 / 8
中国农业科学院科技创新工程项目(CAAS-IEDA-T07);国家自然科学基金面上项目(31672202)
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