不同光质对岩黄连生长发育、细胞结构及有效成分含量的影响OA北大核心CSTPCD
The effects of different light qualities on the growth and development,cell structure and active ingredient content of Corydalis saxicola Bunting
为研究植物工厂中不同光质对岩黄连生长发育、细胞结构和有效成分积累的影响,以岩黄连实生苗为试验材料,采用白、红(R)、蓝(B)LED光源设计8个处理:白光(对照)、蓝光(E1)、R∶B=2∶1(E2)、R∶B=4∶1(E3)、R∶B=6∶1(E4)、R∶B=8∶1(E5)、R:B=10:1(E6)和红光(E7),测定不同处理下植株生长指标及岩黄连碱含量,观察叶片解剖结构.结果表明:红光比例增加对植物生长和成分含量有显著影响,E3处理叶柄数目、主根直径和地上干重显著增加,E4处理可优化根冠比,纯红光促进株高和主根生长.叶绿素a含量在蓝光和红光比例高的E5处理较高,E2处理对叶绿素b、总叶绿素和类胡萝卜素含量影响显著.E4处理根部岩黄连碱含量超过地上部分,E3、E5和E7处理岩黄连总生物碱含量增加.细胞结构观察显示,提高红光比例会导致植物组织结构出现差异:E2处理栅栏组织显著;E3和E7处理栅栏细胞分化整齐;E4和E5处理细胞排列紊乱,叶肉层松散;E6处理上皮细胞萎缩,海绵组织疏松增厚,栅栏组织分化不明显.综上,光质调节对岩黄连植物的生长发育和次生代谢产物积累具有显著的调控作用.
To study the effects of different light qualities on the growth,development,cellular structure,and accumulation of active components in Corydalis saxicola Bunting in a plant factory,the C.saxicola seedlings were used as the experimental material and 8 treatment were designed using white,red(R),and blue(B)LED light sources.White light(control),Blue light(E1),R∶B ratio of 2∶1(E2),R∶B ratio of 4∶1(E3),R∶B ratio of 6∶1(E4),R:B ratio of 8∶1(E5),R∶B ratio of 10:1(E6),Red light(E7).The growth indicators of plants and the content of dehydrocavidine under different treatments were measured and the anatomical structure of the leaves were observed.The results showed that:The increase in red light ratio had a significant impact on plant growth and component content.While E3 treatment significant increased the number of petioles,the diameter of the main root,and the aboveground dry weight;group E4 can optimize the root-to-shoot ratio;while pure red light promoted the growth of plant height and main root length.The content of chlorophyll a was higher in E5 treatment with high proportion of blue light and red light;group E2 had an advantage in the content of chlorophyll b,total chlorophyll,and carotenoids.At group E4,the content of Corydalis alkaloids in the root exceeded that in the aboveground part;the total alkaloid content in groups E3,E5,and E7 increased.Observations of tissue structure showed that an increase in the proportion of red light led to differences in plant tissue structure:group E2 had a significant palisade tissue;groups E3 and E7 had orderly differentiated palisade cells;groups E4 and E5 had disordered cell arrangement and loose mesophyll;group E6 had shrunken epidermal cells,loose and thickened spongy tissue,and unclear palisade tissue differentiation.In summary,light quality regulation has a significant regulation on the growth and development and secondary metabolites accumulation of C.saxicola.
黄丽容;李翠;唐春风;黄燕芬;张占江;郭晓云
广西壮族自治区药用植物园国家中医药传承创新中心/广西药用资源保护与遗传改良重点实验室/广西壮族自治区中药资源智慧创制工程研究中心,南宁 530023
园艺学与植物营养学
岩黄连LED光质生长指标岩黄连碱石蜡切片
Corydalis saxicola BuntingLED light qualitygrowth indicatorsdehydrocavidineparaffin sections
《中国农业大学学报》 2024 (010)
151-160 / 10
广西重点研发计划项目(桂科AB23026092);广西自然科学基金面上项目(2023GXNSFAA026509,2020GXNSFAA159151);广西中医药管理局广西特色药材关键技术研究推广项目(GZKJ2314);广西中医药适宜技术开发与推广项目(GZSY22-02);广西药用资源保护与遗传改良重点实验室项目(KL2022ZZ04,KL2023ZZ09,KL2023KFO4);广西中药材品质创新研究团队项目(GZKJ2305)
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