中国蔬菜Issue(8)：37-46,10.DOI:10.19928/j.cnki.1000-6346.2024.2019
不同组织与逆境胁迫下茄子qRT-PCR内参基因筛选
Screening of Eggplant qRT-PCR Internal Reference Genes under Different Tissues and Adversity Stresses
摘要
Abstract
To screen for suitable internal reference genes in eggplant under different tissues and adversity stresses,UBC,EF-1a,18S,Actin,GAPDH,β-TUB,L33 and EF2,eight candidate internal reference genes were selected in this study under eight experimental conditions,including flood stress,drought stress,cold stress,heat stress,Verticillium dahliae infestation,salt stress,salicylic acid treatment and different tissue sites.The stability of candidate internal reference genes was analyzed using geNorm,NormFinder,ACT,Bestkeeper and RefFinder under eight test conditions.The results showed that Actin was the most stable internal gene among all samples,cold stress,heat stress,Verticillium dahliae infestation and salt stress,β-TUB was the most stable gene expressed under flooding stress,EF2 was the most stable under drought stress,EF-1a was the most stable gene expressed under salicylic acid treatment,and 18S was the most stable among different tissue sites.UBC,L33 and GAPDH were not stably expressed under all treatments.This study provides stable internal reference genes for the study of gene specific expression in different tissues of eggplants and under adversity stress.关键词
茄子/不同组织/逆境胁迫/内参基因Key words
eggplant/different tissues/adversity stress/internal reference genes引用本文复制引用
邓嘉硕,冯程,张泷昊,杨旭..不同组织与逆境胁迫下茄子qRT-PCR内参基因筛选[J].中国蔬菜,2024,(8):37-46,10.基金项目
国家自然科学基金项目(31972395) （31972395）
