中国蔬菜Issue(8)：47-57,11.DOI:10.19928/j.cnki.1000-6346.2024.3045
基于转录组测序的山药NBS-LRR家族基因鉴定和分析
Identification and Analysis of NBS-LRR Family Genes in Dioscorea opposita Based on Transcriptome Sequencing
摘要
Abstract
To explore the role of NBS-LRR resistance genes in response to different fungal infections in'Tiegun'yam,NBS-LRR genes DoRGA2 and DoRGA4 were amplified from the genome of'Tiegun'yam by homologous cloning.RT-qPCR analysis revealed that the infection of the black spot disease pathogenic fungus Alternaria alternata and the endophytic fungus Penicillium halotolerans could induce the up-regulated expression of DoRGA2 and DoRGA4 in different degrees.Meanwhile,RNA-seq was performed to analyze the NBS-LRR family genes of'Tiegun'callus infected by A.alternata and P.halotolerans,the results showed that a total of 47 NBS-LRR family genes were identified,including 5 types of N,NL,CN,CNL and TN,with 25,15,3,3 and 1 gene,respectively.Furthermore,different subfamilies of NBS-LRR proteins had different evolutionary paths,and these proteins were mainly acidic proteins with hydrophilic properties,and were mainly located in the cell membrane or cytoplasm.In addition,among these NBS-LRR family genes,the expressions of TRINITY_DN9146_c0_g1,TRINITY_DN8009_c1_g1,TRINITY_DN49830_c0_g1 and TRINITY_DN39475_c0_g1 were significantly up-regulated after inoculation with A.alternata,while TRINITY_DN18700_c0_g3 was significantly up-regulated after infection with P.halotolerans.关键词
山药/NBS-LRR基因/互隔交链孢/黑斑病/RNA-seqKey words
Dioscorea opposita/NBS-LRR genes/Alternaria alternata/black spot disease/RNA-seq引用本文复制引用
李顺朋,李朝闯,王兰宁,张昊,王瑞飞,李明军,杨清香..基于转录组测序的山药NBS-LRR家族基因鉴定和分析[J].中国蔬菜,2024,(8):47-57,11.基金项目
国家中药材产业技术体系项目(CARS-21),国家重点研发计划资助项目(2023YFD1600603),河南省高等学校重点科研项目(24A210016),中原英才计划-科技创新领军人才项目(224200510011) （CARS-21）
