中国蔬菜Issue(8)：58-64,7.DOI:10.19928/j.cnki.1000-6346.2024.2021
低温预处理对辣椒花药培养及生理指标的影响
Effects of Low Temperature Pretreatment on Anther Culture and Physiological Index in Pepper
摘要
Abstract
In order to further improve the anther culture efficiency of pepper,taking pod pepper XR1 as the experimental material,the effects of pretreatment of flower buds at normal temperature(25 ℃,CK)and low-temperature(0、4、8 ℃)on the efficiency of XR1 anther culture and changes in physiological indicators were studied.The results showed that compared with normal temperature,after low-temperature pretreatment of flower buds,the anther browning rate was significantly reduced,and the induction rate of embryoids was significantly increased.In addition to the significantly reduced MDA content in anthers,the contents of stress-resistant substances(soluble proteins,soluble sugars,Pro)and the activities of antioxidant enzymes(POD,PPO)were significantly increased.The anther browning rate was the lowest under 0 ℃ treatment,the induction rate of cotyledon-shaped embryoids was the best under 4 ℃ treatment,and the induction rate of non-cotyledon-shaped embryoids was the best under 8 ℃ treatment.Among the anthers,except for the highest MDA content under 0 ℃ treatment,the other physiological indicators reached the highest values under 4 ℃ treatment.Correlation analysis showed that there was a negative correlation between the anther browning rate and the induction rate of embryoids(cotyledon-shaped and non-cotyledon-shaped),and there was an extremely significant negative correlation with the MDA content and the strongest correlation,with a correlation coefficient of-0.967.The induction rates of both cotyledon-shaped and non-cotyledon-shaped embryoids were extremely significantly positively correlated with the soluble sugar content in the resistance index and had the strongest correlation,with correlation coefficients of 0.837 and 0.784,respectively.Only cotyledon-shaped embryoids could induce tissue culture seedlings during anther culture,and the pretreatment of flower buds at 4 ℃ had the best effect.The research results can provide a theoretical basis for in-depth analysis of the benefits of low-temperature pretreatment in improving the efficiency of pepper anther culture.关键词
辣椒/花蕾/低温预处理/花药培养/生理指标Key words
pepper/flower buds/low temperature pretreatment/anther culture/physiological indexes引用本文复制引用
丁子健,张俊华,吴丽丽,唐晓玲,蒋清清,程纬韬,杨博智,周书栋..低温预处理对辣椒花药培养及生理指标的影响[J].中国蔬菜,2024,(8):58-64,7.基金项目
湖南省教育厅科学研究项目(22B0214),湖南省蔬菜产业技术体系辣椒育种岗位项目 （22B0214）
