云南番茄潜叶蛾捕食性天敌资源调查及其控害作用分子检测OA北大核心CSTPCD

Yunnan Province is one of the two earliest invaded areas of Tuta absoluta(Meyrick)(Lepidoptera:Gelechiidae)in China.The potential indigenous natural enemies of T.absoluta were investigated,collected,and identified during July to October in tomato planting in 10 prefectures of Yunnan Province.The species of collected natural enemies were identified using DNA barcoding technique and their control efficacy on T.absoluta in tomato fields were evaluated using T.absoluta species-specific PCR(SS-PCR)technique.Our results indicated that there were 36 predatory species detected in tomato fields,belonging to 16 families,5 orders,2 classes.The results of SS-PCR detection shown that 19 species,belonging to 12 families,3 orders,2 classes,fed on T.absoluta.Among the 19 predatory natural enemy species,8 species belong to Insecta and 11 species belong to Arachnida.The percentages of positive detection of T.absoluta were between 5.7%and 100.0%.Of the 19 predatory species,the most widely distributed was Nesidiocoris tenuis Reuter(Hemiptera:Miridae),which was detected in 8 prefectures,followed by Oirus agilis(Flor),Orius sp.(Hemiptera:Anthocoridae),and Coccinella septempunctata L.(Coleoptera:Coccinellidae),which were detected in 5,4,and 5 prefectures,respectively.Pardosa pseudoannulata(Böesenberg et Strand)(Araneae:Lycosidae)were detected in 3 prefectures and the other 14 predatory species were detected only in one or two prefectures.Of the 10 tomato planting prefectures,Zhaotong had the most abundant natural enemy species of T.absoluta,where 8 predatory species were identified,followed by Lijiang and Yuxi,where 6 and 5 predatory natural enemy species were identified,respectively.Four and three predatory natural enemy species were identified in Kunming and Qujing,respectively,two species identified in Dali,Wenshan,and Honghe,and only one species was identified in Chuxiong and Puer.Comprehensive evaluation,based on percentage of positive detection of T.absoluta,dominance of predatory natural enemy population,and distribution frequency,showed that N.tenuis had the greatest potential for control of T.absoluta,followed by O.agilis,C.septempunctata and P.pseudoannulata also had good potential for control of T.absoluta.Nowadays,the occurrence of T.absoluta in tomato fields in some rejoins of China is serious,however,the rich species of natural enemies in Southwestern China can provide reliable natural resource for utilizing domestic natural enemies to manage T.absoluta.