中国生物防治学报2024，Vol.40Issue(4)：948-957,10.DOI:10.16409/j.cnki.2095-039x.2024.02.033
贝莱斯芽胞杆菌C1B1生防活性及促生作用研究
Biocontrol Activity and Growth Promoting Effect of Bacillus velezensis C1B1
摘要
Abstract
Strain C1B1 is an endophytic bacterial strain of industrial hemp obtained earlier.Morphological,physiological and biochemical characteristics of the strains and 16S rRNA and gyrA gene sequences were analyzed to clarify the taxonomic status of the strains.The antagonistic and growth-promoting properties were assessed by plate standoff method,potting and growth-promoting experiments.The results showed that strain C1B1 belongs to Bacillus velezensis,and could inhibit pepper southern blight(Sclerotium rolfsii)with the efficacy up to 87.22%in the plate,and the potting efficacy of the strain was 44.4%.Meanwhile,this strain had good inhibitory effect on seven kinds of plant pathogens such as grapefruit anthracnose Colletotrichum gloeosporioides,lucerne wilt Fusarium oxysporum,etc.The germination rate of pepper seeds increased by 20.1%and 22.3%after treatment with C1B1 fermentation broth and aseptic supernatant,respectively.The plant height and stem thickness of pepper plants increased by 22.6%and 29.1%,15.7%and 18.1%,respectively.In conclusion,strain C1B1 has a good antagonistic effect against white silk disease and other pathogens of chili pepper,and has a growth-promoting effect on chili pepper crops,which has a good potential for biocontrol application.关键词
贝莱斯芽胞杆菌/辣椒白绢病/生物防治/盆栽试验/促生作用Key words
Bacillus velezensis/pepper white silk disease/biological control/pot experiment/growth-promoting effect分类
农业科技引用本文复制引用
王芳,宁丽平,邓稳桥,秦菁菁,许秀美,孙正祥,程毅..贝莱斯芽胞杆菌C1B1生防活性及促生作用研究[J].中国生物防治学报,2024,40(4):948-957,10.基金项目
中国农业科学院创新工程(ASTIP-IBFC-04) （ASTIP-IBFC-04）
湖南省农业种质资源普查保护利用项目(XCN202137) （XCN202137）
湖南省植保植检站植物防疫防控科研项目(HNZB202103) （HNZB202103）
