中国实用妇科与产科杂志2024，Vol.40Issue(8)：849-854,6.DOI:10.19538/j.fk2024080116
定坤丹通过microRNA-30d-5p靶向调控卵巢组织Smad 2治疗多囊卵巢综合征模型大鼠的机制研究
Study on the mechanism of Dingkundan targeting and regulating Smad2 in ovarian tissue through microRNA-30d-5p to treat PCOS model rats
摘要
Abstract
Objective To observe the effects of Dingkundan on ovarian tissue morphology,serum sex hormone levels,transforming growth factor-β1(TGF-β1),Smad 2,Smad 3,and microRNA-30d-5p expression in polycystic ovary syn-drome(PCOS)model rats,and to further explore the mechanism of Dingkundan's targeted regulation of Smad 2 in ovari-an tissue through microRNA-30d-5p in treating PCOS model rats.Methods DHEA-induced PCOS rat models were treated with low[1.125 g/(kg·d)],medium[2.25 g/(kg·d)]and high[4.5 g/(kg·d)]doses of Dingkundan.Enzyme linked immunosorbent assay(ELISA),hematoxylin-eosin(HE)staining,immunohistochemistry,real time polymerase chain reaction(RT-PCR)and Western blot were used to detect the ovarian histomorphology,serum sex hormone levels,microRNA-30d-5p expressions,as well as protein and mRNA expressions of TGF-β1,Smad 2 and Smad 3.Results Compared with model group,all Dingkundan treatment groups significantly promoted follicles of all levels and corpus luteum in the ovaries,downregulated the levels of estradiol(E2),luteinizing hormone(LH)and testosterone(T)while in-creasing follicle stimulating hormone(FSH)level,the differences being statistically significant;both protein and mRNA expressions of TGF-β1,Smad 2 and Smad 3 decreased while microRNA-30d-5p expressions increased,both dif-ferences being statistically significant.Conclusions The DHEA-induced PCOS rat model is an ideal animal model for studying ovulation disorders in PCOS.Dingkundan has a certain therapeutic effect on PCOS rats,and its mechanism may be to target and regulate the expression level of Smad 2 in ovarian tissue through microRNA-30d-5p.关键词
多囊卵巢综合征/定坤丹/转化生长因子β1/Smad 2/Smad 3/microRNA-30d-5pKey words
polycystic ovary syndrome/Dingkundan/transforming growth factor β1/Smad 2/Smad 3/microRNA-30d-5p分类
医药卫生引用本文复制引用
陈兰,杨波,罗立波,颜晓静,王地均,蔡佳丽,尹春燕..定坤丹通过microRNA-30d-5p靶向调控卵巢组织Smad 2治疗多囊卵巢综合征模型大鼠的机制研究[J].中国实用妇科与产科杂志,2024,40(8):849-854,6.基金项目
国家自然科学基金-青年科学基金(82104905) （82104905）
常州市孟河医派人用经验研究与转化重点实验室(CM20223006) （CM20223006）
南京中医药大学技术开发项目(2017005) （2017005）
