宿主蛋白RPSA在非洲猪瘟病毒复制过程中的作用研究OA北大核心CSTPCD

This study aimed to investigate the impact of host protein RPSA the replication of African swine fever virus(ASFV)and the interaction between viral B602L protein and RPSA.The recombi-nant RPSA plasmid pcDNA3.1-Flag-RPSA was constructed and transfected into HET 293 cells,and the sub-cellular localization of B602L and RPSA proteins was examined through immunofluorescence assay(IFA),as well as their interaction was validated through immunoprecipitation(Co-IP).Additionally,the expr-essed differences of RPSA protein during ASFV infected different time points in IPAM cells were ana-lyzed through quantitative real-time PCR(qPCR)and Western-blot.Furthermore,the impact of RPSA on the ASFV replication process was analyzed by over-expression and interfering of RPSA expression in IPAM cells.The results showed that the expression of RPSA protein gradually increased over time fol-lowing ASFV infection in IPAM cell over-expression of RPSA could inhibit viral replication,while in-terference of RPSA leads to enhance of viral replication.Moreover,the results of Co-IP and subcellular localization were also confirmed that the interaction between viral B602L protein and host RPSA pro-tein.In summary,this study revealed the critical role of host RPSA protein in the ASFV replication pro-cess,providing an important theoretical basis for further mechanism research.These findings con-tribute to a deeper understanding of the infection mechanism of ASFV infection and provide new clues for disease control and treatment.