中国兽医科学2024，Vol.54Issue(9)：1149-1156,8.DOI:10.16656/j.issn.1673-4696.2024.0141
宿主蛋白RPSA在非洲猪瘟病毒复制过程中的作用研究
Role of host protein RPSA in the replication of African swine fever virus
摘要
Abstract
This study aimed to investigate the impact of host protein RPSA the replication of African swine fever virus(ASFV)and the interaction between viral B602L protein and RPSA.The recombi-nant RPSA plasmid pcDNA3.1-Flag-RPSA was constructed and transfected into HET 293 cells,and the sub-cellular localization of B602L and RPSA proteins was examined through immunofluorescence assay(IFA),as well as their interaction was validated through immunoprecipitation(Co-IP).Additionally,the expr-essed differences of RPSA protein during ASFV infected different time points in IPAM cells were ana-lyzed through quantitative real-time PCR(qPCR)and Western-blot.Furthermore,the impact of RPSA on the ASFV replication process was analyzed by over-expression and interfering of RPSA expression in IPAM cells.The results showed that the expression of RPSA protein gradually increased over time fol-lowing ASFV infection in IPAM cell over-expression of RPSA could inhibit viral replication,while in-terference of RPSA leads to enhance of viral replication.Moreover,the results of Co-IP and subcellular localization were also confirmed that the interaction between viral B602L protein and host RPSA pro-tein.In summary,this study revealed the critical role of host RPSA protein in the ASFV replication pro-cess,providing an important theoretical basis for further mechanism research.These findings con-tribute to a deeper understanding of the infection mechanism of ASFV infection and provide new clues for disease control and treatment.关键词
非洲猪瘟病毒/B602L/RPSA/相互作用Key words
ASFV/B602L/RPSA/interaction分类
农业科技引用本文复制引用
邵程祥,张忠辉,孙华林,赵亚茹,杨吉飞,关贵全,殷宏,武军元,牛庆丽..宿主蛋白RPSA在非洲猪瘟病毒复制过程中的作用研究[J].中国兽医科学,2024,54(9):1149-1156,8.基金项目
国家自然科学基金面上项目(32072830) （32072830）
国家重点研发计划项目(2021YFD1800101) （2021YFD1800101）
农业科技创新工程项目(ASTIP,CAAS-ASTIP-2016-LVRI) （ASTIP,CAAS-ASTIP-2016-LVRI）
甘肃省科技重大专项(22ZD6NA001) （22ZD6NA001）
甘肃省基础研究创新群体项目(22JR5RA024) （22JR5RA024）
甘肃省优秀博士生项目(22JR5RA025) （22JR5RA025）
