中国兽医科学2024，Vol.54Issue(9)：1175-1181,7.DOI:10.16656/j.issn.1673-4696.2024.0153
表达基因Ⅰ亚型禽呼肠孤病毒σB和σC的重组4型禽腺病毒的拯救及鉴定
Rescue and identification of recombinant fowl adenovirus 4 expressingσB and σC of genotype-Ⅰ subcluster avian reovirus
摘要
Abstract
Inorder to obtain a recombinant fowl adenovirus 4(FAdV-4)strain that could express the σB and σC of avian reovirus Ⅰ-sub(ARV GCI-sub),a novel Fosmid clay rescue system was used to rescue the virus using the fowl adenovirus rHN20 as the vector and the recombinant virus rHN20-r1966-ARV GCI-sub ex-pressing σB and σC proteins was constructed.The successful rescue of recombinant virus was proved by PCR,indirect immunofluorescence assay(IFA),Wes tern-blot,and the kinetics of replication in vi tro.The results showed that σB and σC gene of ARV GCI sub were detected in the LMH cells infected with recombi-nant virus by the PCR,Fiber protein of FAdV-4,σB and σC protein of ARV GCI sub were detected in the LMH cells infected with recombinant virus by the Western-blot experiment,and σB and σC protein of ARV GCI sub were specifically detected in the LMH cells infected with recombinant virus by the IFA experiment.The recombinant virus showed no significant difference in replication ability between the recombinant virus and the parent virus,indicating successful rescue of recombinant virus rHN20-r1966-ARV GCI-sub.The construction of recombinant virus rHN20-r1966-ARV GCI-sub provides technical support for ARV pre-vention and control vaccines targeting σB and σC protein of ARV generation at the same time,and lays a foundation for the combined prevention and control of ARV and FAdV-4.关键词
基因Ⅰ亚型禽呼肠孤病毒/4型禽腺病毒/σB/σ-C/重组病毒Key words
avian reovirus Ⅰ-sub/fowl adenovirus 4/σ B/σ C/recombinant virus分类
农业科技引用本文复制引用
许壮壮,张艳萍,崔红玉,刘永振,段雨路,王牟平,高玉龙,郭茹,王素艳,高立,陈运通,张涛,高宁玉,吴龙波,祁小乐..表达基因Ⅰ亚型禽呼肠孤病毒σB和σC的重组4型禽腺病毒的拯救及鉴定[J].中国兽医科学,2024,54(9):1175-1181,7.基金项目
国家重点研发计划项目(2022YFF0710500) （2022YFF0710500）
国家肉鸡产业技术体系项目(CARS-41) （CARS-41）
