中国兽医科学2024，Vol.54Issue(9)：1232-1239,8.DOI:10.16656/j.issn.1673-4696.2024.0146
一起牛呼吸道综合征病原分析和鉴定
Identification and pathogen analysis of bovine respiratory disease complex
摘要
Abstract
Bovine respiratory disease complex(BRDC)is a multi-symptom disease caused by various pathogens and environmental factors,resulting in significant economic losses for cattle farming industry.In March 2023,BRDC broke out in a beef cattle farm in Shanxi province.To detect the pathogens,4 swabs from the deep nasal cavity of infected cattle and 4 tissue samples from dead cattle were collected.These samples were detected by qPCR and RT-qPCR for 9 pathogens related to BRDC,includ-ing M.bovis,bovine parainfluenza virus type 3(BPIV-3),infectious bovine rhinotracheitis virus(I-BRV),bovine coronavirus(BCoV),bovine respiratory syncytial virus(BRSV),P.multocida,M.haemolyti-ca,Arcanobacterium pyogenes(A.pyogenes)and Histophilus somnus(H.somnus).The results indicated that the positive rate of M.bovis,P.multocida and H.somnus was 75％,the positive rate of M.haemolytica was 50％,A.pyogenes was 62％,the positive rates of BCoV were 25％.It was proved that the BRDC was caused by the mixed infection of the above 6 pathogens.The pathogens were isolated and identified to obtain 1 strain of M.hemolytia and 1 strain of P.multocida,and the drug susceptibility test was carried out.The results provided a reference for the prevention and control of calf respiratory diseases and the formu-lation of targeted treatment measures.关键词
牛呼吸道疾病综合征/病原检测/混合感染/病原分离鉴定/药敏试验Key words
BRDC/pathogen detection/mixed infection/pathogen isolation and identification/drug sensitivity tests分类
农业科技引用本文复制引用
刘永杰,颜新敏,刘萍,张伟,曹小安,兰喜,尚佑军,何继军..一起牛呼吸道综合征病原分析和鉴定[J].中国兽医科学,2024,54(9):1232-1239,8.基金项目
"十四五"国家重点研发计划项目(2021YFD1800500) （2021YFD1800500）
