中国兽医科学2024，Vol.54Issue(9)：1248-1252,5.DOI:10.16656/j.issn.1673-4696.2024.0144
表达绿色荧光蛋白的重组伪狂犬病病毒构建及在中和试验中应用
Construction of recombinant pseudorabies virus expressing green fluorescent protein and its application in neutralization test
摘要
Abstract
In order to optimize the viral serum neutralizing antibody test method and reduce the cycle time of traditional viral neutralizing antibody test,a pseudorabies gE gene deletion virus PRV-△gE-AcGFP1 expressing green fluorescent protein was constructed by homologous recombination technology,and used it as a test virus for determination of neutralizing antibody potency by performing the viral serum neutralizing antibody test on gE antibody-negative sera.The results showed that there was no significant difference between the recombinant virus and the traditional method in determining the titer of neutralizing antibody.The results show that PRV-△gE-AcGFP1 could be used as test virus for serum neutralizing antibody assay,providing a new option for performing convenient,accurate and short-cycle serum neutralizing antibody tests.关键词
伪狂犬病病毒/同源重组/绿色荧光蛋白/中和试验Key words
pseudorabies virus/homologous recombination/green fluorescent protein/neutraliza-tion test分类
农业科技引用本文复制引用
任晓祥,刘立辉,肖娜,张帅,武月,赵云环,刘涛,左玉柱,范京惠..表达绿色荧光蛋白的重组伪狂犬病病毒构建及在中和试验中应用[J].中国兽医科学,2024,54(9):1248-1252,5.基金项目
河北省农业产业技术体系生猪创新团队项目(HBCT2024220201) （HBCT2024220201）
河北省农业产业技术体系生猪创新团队项目(HBCT2024220401) （HBCT2024220401）
