中国兽医科学2024，Vol.54Issue(9)：1259-1266,8.DOI:10.16656/j.issn.1673-4696.2024.0165
HIF-2α、LP及LEPR在不同年龄牦牛肺组织中的表达
Expression of HIF-2α,LP and LEPR in lung tissues of yaks at different ages
摘要
Abstract
The aim of this study was to investigate the distribution characteristics of hypoxi-a-inducible factor 2α(HIF-2α),leptin(LP)and its receptor(LEPR)in the lungs of yaks of different ages,and the possible roles they may play in the growth and development of yak lungs.In this experi-ment,immunohistochemical staining,qRT-PCR and Western-blot were used to investigate the distribution and expression levels of HIF-2α,LP and LEPR in the lungs of yaks of different ages.Immunohistochem-istry showed that HIF-2α was mainly expressed in the terminal bronchial epithelial cells and the ac-companying pulmonary artery endothelium of yak lungs at different ages;LP and LEPR had similar sites of expression,and were mainly distributed in the bronchial epithelium at all levels of the lungs of yak lungs at different ages,pulmonary artery endothelium,and alveolar epithelium.qRT-PCR showed that the expression level of HIF-2α was significantly higher(P＜0.05)than that of 6-year-old,3-year-old,and 6-year-old yaks in 3-year-old and 6-year-old yak lungs.The qRT-PCR results showed that the expression of HIF-2α gene in the lungs of 3-day-old yaks was significantly higher than that in the lungs of 6-month-old,3-year-old and 6-year-old yaks(P＜0.05),the expression of LP gene in the lungs of 3-day-old and 6-year-old yaks was the highest and significantly higher than that in 6-month-old yaks(P＜0.05),and the expression of LEPR gene in the lungs of 3-day-old yaks was significantly higher than that in the lungs of 6-month-old,3-year-old and 6-year-old yaks(P＜0.05).The results of Western-blot showed that LP protein had the highest expression at 3 days of age and was significantly higher than other ages(P＜0.05).LEPR and HIF-2α proteins were significantly higher at 3 days of age than at 6 months of age and 3 years of age(P＜0.05),and the difference was not significant between them and 6 years of age(P＞0.05).The results indicated that HIF-2α,LP and LEPR might play important roles in the development of yak lungs,and the present study provides some basic information for further investigating the mech-anism of lung adaptation in yak under low oxygen environment.关键词
牦牛/肺/瘦素(LP)/瘦素受体(LEPR)/低氧诱导因子(HIF-2α)Key words
yak/lung/leptin(LP)/leptin receptor(LEPR)/hypoxia inducible factors-2α(HIF-2α)分类
农业科技引用本文复制引用
翟国亮,崔燕,余四九,赵鹏飞,魏鹏强,卢凯,祁正满,卢增华..HIF-2α、LP及LEPR在不同年龄牦牛肺组织中的表达[J].中国兽医科学,2024,54(9):1259-1266,8.基金项目
国家自然科学基金项目(32372974) （32372974）
