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广西地区犬和猫肠道病毒的病原学调查研究OA北大核心

Etiology Investigation on Enteroviruses from Dogs and Cats in Guangxi

中文摘要英文摘要

为了解引起广西地区犬和猫腹泻等肠道病原的分布情况,并探究犬和猫新型肠道病毒感染的临床特征,为临床中及早诊治和防控肠道疾病提供参考依据,本试验采集宠物犬和猫粪便拭子样品394份(犬样品172份,猫样品222份),并通过聚合酶链式反应(PCR)或反转录酶-聚合酶链式反应(RT-PCR)筛查犬细小病毒(CPV)、猫瘟病毒(FPV)和查帕马细小病毒(ChPV)等13种主要犬和猫肠道病毒,并分析肠道病毒阳性率与年龄、性别、免疫状况和临床症状之间的相关性.结果显示,犬粪便样品中总阳性样品数为49份,占比28.49%;其中CPV的阳性率最高,达到15.12%,临床症状为典型的肠道症状;其次,犬粪便样品中还检测到犬冠状病毒(CCoV,4.65%)、猫杯状病毒(FCV,4.07%)、嵴病毒(KoV,4.07%)、博卡病毒(BoV,3.49%)、犬瘟热病毒(CDV,2.91%)和布法病毒(BuV,0.58%);其中11份粪便样品混合感染了2种及以上种类的肠道病毒,CPV感染占10份.幼龄犬对CCoV和FCV比较易感;各肠道病毒阳性率与性别无相关性,部分肠道病毒阳性率与免疫状况和临床症状有相关性.猫粪便样品中总阳性样品数为32份,占比14.41%;FPV的阳性率最高,达到8.11%;其次,猫粪便样品中还检测到新型肠道病毒,包括FCV(4.05%)、ChPV(1.35%)、BoV(1.35%)、BuV(0.45%)和猫冠状病毒(FCoV,0.45%);其中混合感染样品仅4份,而FCV感染占3份.FPV和BoV阳性率与猫的年龄和免疫状况存在相关性,各肠道病毒阳性率与猫的性别和临床症状无明显相关性.值得关注的是,犬和猫粪便中均可检测到A型流感病毒(IAV),阳性率分别为2.91%和0.45%.综上表明,CPV和FPV仍是引起犬和猫肠道疾病的主要病原,同时也是混合感染的重要组成;多种新型肠道病毒的阳性率虽然不高,但仍是引起肠道或肠外疾病中不可忽视的病原;IAV的检出再次强调了犬和猫作为"中间宿主"的重要作用.本试验结果不仅为犬和猫肠道传染病的防控提供预警,也为切断宠物-人IAV传播提供重要参考.

To understand the distribution of enteric pathogens causing diarrhea and other gastrointestinal diseases in dogs and cats in Guangxi and to explore the clinical characteristics of novel enteroviruses infections in these animals,providing a reference for early diagnosis and prevention of enteric diseases,this study collected 394 fecal swab samples from pet dogs and cats(172 dog samples,222 cat samples),and screened for 13 major enteroviruses in dogs and cats,including canine parvovirus(CPV),feline panleukopenia virus(FPV),and Chaphamaparvoviruses(ChPV),using polymerase chain reaction(PCR)or reverse transcription-polymerase chain reaction(RT-PCR).The correlation between enteroviruses positivity rates and factors such as age,gender,immunization status,and clinical symptoms was analyzed.The results showed that 49 dog fecal samples were positive,accounting for 28.49%.CPV had the highest positivity rate at 15.12%,with clinical symptoms typical of enteric infection.Other detected viruses included canine coronavirus(CCoV,4.65%),feline calicivirus(FCV,4.07%),kobuvirus(KoV,4.07%),bocavirus(BoV,3.49%),canine distemper virus(CDV,2.91%),and bufavirus(BuV,0.58%).Eleven samples showed co-infection with two or more types of enteroviruses,with CPV involved in ten of these cases.Young dogs were more susceptible to CCoV and FCV.Positivity rates of enteroviruses showed no correlation with gender,but some were correlated with immunization status and clinical symptoms.In cat fecal samples,32 were positive,accounting for 14.41%.FPV had the highest positivity rate at 8.11%.Novel enteroviruses detected included FCV(4.05%),ChPV(1.35%),BoV(1.35%),BuV(0.45%),and feline coronavirus(FCoV,0.45%).Co-infections were observed in only four samples,with FCV detected in three of these.FPV and BoV positivity rates correlated with age and immunization status,but no significant correlation with gender or clinical symptoms was found.Notably,influenza A virus(IAV)was detected in both canine and feline feces,with positivity rates of 2.91%and 0.45%,respectively.In conclusion,CPV and FPV remain the primary pathogens causing enteric diseases in dogs and cats and are significant components of co-infections.Although the positivity rates of several novel enteroviruses are not high,they are still noteworthy pathogens causing enteric or extraintestinal diseases.The detection of IAV emphasizes the crucial role of dogs and cats as"intermediate hosts."These results not only provide an early warning for the prevention and control of canine and feline enteric infectious diseases but also offer important insights into preventing pet-to-human transmission of IAV.

黎波宏;黄伟坚;陈樱;黄馨;何庆元;方友桥;满婷婷;陈建材;周华波;韦祖樟;欧阳康

广西大学动物科学技术学院,广西南宁 530004广西大学动物科学技术学院,广西南宁 530004||广西壮族自治区兽用生物制品工程研究中心,广西南宁 530004||广西畜禽繁育与疾病防控重点实验室,广西南宁 530004南宁市华波宠物医院,广西南宁 530004

畜牧业

犬和猫粪便样品肠道病毒混合感染流行病学调查

fecal samples of dog and catenterovirusco-infectionepidemiological investigation

《中国兽医杂志》 2024 (008)

24-31 / 8

广西自然科学基金项目(2021GXNSFAA220070);大学生创新创业训练计划项目(202110593015)

10.20157/j.cnki.zgsyzz.2024.08.004

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