中国兽医杂志2024，Vol.60Issue(8)：24-31,8.DOI:10.20157/j.cnki.zgsyzz.2024.08.004
广西地区犬和猫肠道病毒的病原学调查研究
Etiology Investigation on Enteroviruses from Dogs and Cats in Guangxi
摘要
Abstract
To understand the distribution of enteric pathogens causing diarrhea and other gastrointestinal diseases in dogs and cats in Guangxi and to explore the clinical characteristics of novel enteroviruses infections in these animals,providing a reference for early diagnosis and prevention of enteric diseases,this study collected 394 fecal swab samples from pet dogs and cats(172 dog samples,222 cat samples),and screened for 13 major enteroviruses in dogs and cats,including canine parvovirus(CPV),feline panleukopenia virus(FPV),and Chaphamaparvoviruses(ChPV),using polymerase chain reaction(PCR)or reverse transcription-polymerase chain reaction(RT-PCR).The correlation between enteroviruses positivity rates and factors such as age,gender,immunization status,and clinical symptoms was analyzed.The results showed that 49 dog fecal samples were positive,accounting for 28.49％.CPV had the highest positivity rate at 15.12％,with clinical symptoms typical of enteric infection.Other detected viruses included canine coronavirus(CCoV,4.65％),feline calicivirus(FCV,4.07％),kobuvirus(KoV,4.07％),bocavirus(BoV,3.49％),canine distemper virus(CDV,2.91％),and bufavirus(BuV,0.58％).Eleven samples showed co-infection with two or more types of enteroviruses,with CPV involved in ten of these cases.Young dogs were more susceptible to CCoV and FCV.Positivity rates of enteroviruses showed no correlation with gender,but some were correlated with immunization status and clinical symptoms.In cat fecal samples,32 were positive,accounting for 14.41％.FPV had the highest positivity rate at 8.11％.Novel enteroviruses detected included FCV(4.05％),ChPV(1.35％),BoV(1.35％),BuV(0.45％),and feline coronavirus(FCoV,0.45％).Co-infections were observed in only four samples,with FCV detected in three of these.FPV and BoV positivity rates correlated with age and immunization status,but no significant correlation with gender or clinical symptoms was found.Notably,influenza A virus(IAV)was detected in both canine and feline feces,with positivity rates of 2.91％and 0.45％,respectively.In conclusion,CPV and FPV remain the primary pathogens causing enteric diseases in dogs and cats and are significant components of co-infections.Although the positivity rates of several novel enteroviruses are not high,they are still noteworthy pathogens causing enteric or extraintestinal diseases.The detection of IAV emphasizes the crucial role of dogs and cats as"intermediate hosts."These results not only provide an early warning for the prevention and control of canine and feline enteric infectious diseases but also offer important insights into preventing pet-to-human transmission of IAV.关键词
犬和猫粪便样品/肠道病毒/混合感染/流行病学调查Key words
fecal samples of dog and cat/enterovirus/co-infection/epidemiological investigation分类
农业科技引用本文复制引用
黎波宏,黄伟坚,陈樱,黄馨,何庆元,方友桥,满婷婷,陈建材,周华波,韦祖樟,欧阳康..广西地区犬和猫肠道病毒的病原学调查研究[J].中国兽医杂志,2024,60(8):24-31,8.基金项目
广西自然科学基金项目(2021GXNSFAA220070) （2021GXNSFAA220070）
大学生创新创业训练计划项目(202110593015) （202110593015）
