2种诱导溃疡性结肠炎动物模型方法的比较OA北大核心

In order to compare the effectiveness of inducing ulcerative colitis in mice through rectal administration or free drinking to dextran sulfate sodium(DSS)solution,this study utilized 30 male C57BL/6J mice,randomly divided into the Normal control group(WT group),3％DSS free drinking group(3％DSS group),and 6 g/(kg·bw)DSS rectal administration group[6 g/(kg·bw)DSS group],with 10 mice in each group.The models were induced continuously for 7 days.Weigh the mice daily to calculate the rate of weight gain and assess the disease activity index(DAI)for the mice.On the 8 th day post-modeling,mice were dissected,and the colon was isolated,the colon length was measured,H.E.staining was performed to observe the histopathological changes and assign scores,and immunohistochemistry was conducted to observe the expression of Mucin-2(MUC2)in colonic epithelial tissues,and H-score was calculated.Spleen was isolated,weighed,and spleen index was calculated.The results showed that compared with the WT group,mice in the DSS-induced model groups exhibited extremely significant decrease in body weight and colonic epithelial tissue MUC2 H-score(P＜0.01),while DAI scores,histopathological scores,and spleen indexes extremely significant increase(P＜0.01),and colon length extremely significant decrease(P＜0.01).Comparing with the 3％DSS group,mice in the 6 g/(kg·bw)DSS group had significant decrease in body weight and colonic epithelial tissue MUC2 H-score(P＜0.05),significantly increased DAI scores and spleen indexes(P＜0.05),and extremely significant higher histopathological score(P＜0.01),with significant decrease in colon length(P＜0.05).The results suggest that both free drinking of 3％DSS and rectal administration of 6 g/(kg·bw)DSS can establish an ulcerative colitis model in mice,with the 6 g/(kg·bw)DSS model showing more severe and consistent symptoms with a lower DSS dosage.