中国兽医杂志2024，Vol.60Issue(8)：54-61,8.DOI:10.20157/j.cnki.zgsyzz.2024.08.009
鸡呼肠孤病毒基因Ⅰ型变异株的分离鉴定和致病性分析
Isolation,Identification and Pathogenicity Analysis of Avian Reovirus Genotype Ⅰ Variants
许明清 1郭明 1胡峰 2于可响 2李玉峰 2汪建华 1马芹 1刘丹丹 1张中波 1赵杰 1石艳红 1唐代萍 1王胜1
作者信息
- 1. 山东和康源生物育种股份有限公司,山东济南 250100||家禽育种与疾病预防控制济南市工程研究中心,山东济南 250100
- 2. 山东省农业科学院家禽研究所,山东济南 250023
- 折叠
摘要
Abstract
This study aimed to investigate the pathogen responsible for leg problems observed in broiler breeders at a chicken farm in Shandong Province,and to analyze its pathogenicity.Samples were collected from the affected chicken farm,and the investigation included embryonic chicken isolation,cell passage,reverse transcription-polymerase chain reaction(RT-PCR)detection,sequence analysis,pathogenicity tests on commercial broiler chickens,and immunoprotection assays.The results revealed the isolation of a strain of avian reovirus(ARV),named ARV-LL-2020,which exhibited 100％mortality in embryonic chickens and strong adaptability in Leghorn male hepatoma cell line(LMH).The ARV-LL-2020 strain belongs to genotype Ⅰ,similar to the classical S1 133 vaccine strain,with 73.6％nucleotide homology and 59.1％amino acid homology in the major antigen σC gene.In commercial broiler chickens,ARV-LL-2020 replicated symptoms identical to those observed in actual production settings.The commercial attenuated live vaccine strain S1 133 provided only 50％protection against ARV-LL-2020.These findings indicate that ARV-LL-2020 strain causing chicken leg problems is a variant strain of avian reovirus genotype Ⅰ,and the current commercial attenuated live vaccine S1 133 does not provide complete protection against it.关键词
鸡呼肠孤病毒/基因Ⅰ型/分离鉴定/致病性Key words
avian reovirus/genotype Ⅰ/isolation and identification/pathogenicity分类
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许明清,郭明,胡峰,于可响,李玉峰,汪建华,马芹,刘丹丹,张中波,赵杰,石艳红,唐代萍,王胜..鸡呼肠孤病毒基因Ⅰ型变异株的分离鉴定和致病性分析[J].中国兽医杂志,2024,60(8):54-61,8.
