中国兽医杂志2024，Vol.60Issue(8)：125-132,8.DOI:10.20157/j.cnki.zgsyzz.2024.08.019
麻杏石甘散对患呼吸道疾病犊牛上呼吸道菌群的影响
Effects of Maxing Shigan Powder on the Upper Respiratory Tract Microbiota in Calves with Bovine Respiratory Diseases
摘要
Abstract
To investigate the effects of Maxing Shigan Powder on the upper respiratory tract microbiota in calves with bovine respiratory disease(BRD),this study selected 10 naturally infected BRD calves.The calves were administered Maxing Shigan Powder orally at a dosage of 0.75 g/(kg·bw·d)for 7 consecutive days.Clinical symptoms and clinical symptom scores were observed and recorded on days 0,3,5,and 7 of treatment to assess clinical efficacy.Serum was collected on days 0 and 7 to measure concentrations of acute phase proteins[haptoglobin(HP)and serum amyloid A(SAA)].Nasal swabs were collected on days 0 and 7 for 16S rDNA high-throughput sequencing to analyze α-diversity,β-diversity,microbial composition,and differences.The results showed that compared to day 0,the total clinical symptom scores of BRD calves significantly decreased on days 0,3,5,and 7 after Maxing Shigan Powder treatment(P＜0.05),and the scores dropped below 5 on day 5.On day 7 of treatment,the cure rate of the diseased calves was 60％,and the effective rate was 40％,the serum concentrations of HP and SAA significantly decreased(P＜0.05),and there were no significant differences in α-diversity and β-diversity of the upper respiratory tract microbiota(P＞0.05).The main dominant phyla in the upper respiratory tract of the diseased calves were Proteobacteria,Tenericutes,Bacteroidetes,Fusobacteria,and Actinobacteria.The main dominant genera were Pseudomonas,Rahnella,Stenotrophomonas,Mycoplasma,and Psychrobacter.Compared to day 0,the relative abundances of Pseudomonas and Actinomycetales_unclassified extremely significant increased(P＜0.01)on day 7,while the relative abundances of Enterobacteriaceae_unclassified,Erwinia,and Enterobacter significantly decreased(P＜0.05).The relative abundance of Rahnella extremely significant decreased(P＜0.01).These results indicate that administering Maxing Shigan Powder for 7 consecutive days can effectively alleviate clinical symptoms in BRD calves,significantly reduce the relative abundance of harmful bacteria such as Rahnella and Enterobacter in the upper respiratory tract,and may play a positive role in restoring the health of BRD calves.This study provides a reference for the clinical application of Maxing Shigan Powder in cattle.关键词
麻杏石甘散/牛呼吸道疾病/犊牛/上呼吸道菌群Key words
Maxing Shigan powder/bovine respiratory disease/calves/upper respiratory tract microbiota分类
农业科技引用本文复制引用
张力本,庞敏,陈娅婷,黄逸馨,余树民,曹随忠..麻杏石甘散对患呼吸道疾病犊牛上呼吸道菌群的影响[J].中国兽医杂志,2024,60(8):125-132,8.基金项目
四川省区域创新合作项目(2023YFQ0078) （2023YFQ0078）
重庆市教委科学技术研究项目(KJZD-M202303501) （KJZD-M202303501）
