中国现代医学杂志2024，Vol.34Issue(16)：26-32,7.DOI:10.3969/j.issn.1005-8982.2024.16.005
无需缝合外科嵌入式小鼠原位肝癌肿瘤模型的复制及应用价值
Establishment and application value of mouse model of hepatocellular carcinoma via sutureless surgical orthotopic implantation
摘要
Abstract
Objective To explore the application of sutureless surgical orthotopic implantation in the establishment of mouse model of hepatocellular carcinoma(HCC)and to assess the value of this model in tumor biology researches.Methods Hepa 1-6 cells were cultured in vitro to an appropriate density,and C57 mice were subjected to anesthesia using a small animal all-in-one anesthesia system.In this study,three techniques,including the cellular method,the hanging method and the implantation method,were used to replicate the orthotopic transplantation hepatocellular carcinoma model.The operative duration,and the recovery time and the survival rate of mice were compared.In addition,the process of tumor formation was dynamically monitored by magnetic resonance imaging(MRI),and the tumor formation rate,the single tumor rate,and the abdominal wall tumor implantation rate were compared among the three methods.The structural and pathological features of the tumor tissues were evaluated by pathological examinations.Results The operative duration of the cellular method was shorter than that of the hanging method(P＜0.05),and that of the implantation method was shorter than that of the hanging method(P＜0.05).There was no difference in the operative duration between the cellular method and the implantation method(P＞0.05).The recovery time of mice in the cellular method group was shorter than that in the hanging method group(P＜0.05),and that in the implantation method group was shorter than that in the hanging method group(P＜0.05).There was no difference in the recovery time of mice between the cellular method group and the implantation method group(P＞0.05).The MRI exhibited that the tumors in the hanging method group grew in a nodular pattern,with septa and roundish artifacts,and that parts of the tumors were not well visualized.The tumors in the cellular method group grew irregularly and in a lamellar pattern,with multiple lesions observed.The tumors in the implantation method group grew in a lump-like,uniform manner with relatively clear margins.The mean tumor volume 7 days after modeling in the cellular method group was larger than that in the hanging method group(P＜0.05),and that in the implantation method group was smaller than that in the hanging method group(P＜0.05).There was no difference in the mean tumor volume 7 days after modeling between the cellular method group and the implantation method group(P＞0.05).The mean tumor volume 21 days after modelling was not different among the three groups(P＞0.05).The mean tumor weight was also not different across the groups(P＞0.05).There were differences in the single tumor rate and the abdominal wall tumor implantation rate among the groups(P＜0.05),and the single tumor rate in the implantation method group was higher than that in the cellular method group(P＜0.05).There was no difference in the tumor formation rate among the groups(P＞0.05).The HE staining demonstrated that tumor cells in all groups were distributed in a nest-like pattern and arranged closely with deeply stained nuclei showing mitotic figures,and that the tumors infiltrated liver tissues,consistent with the pathological characteristics of hepatocellular carcinoma.Conclusions The mouse model of hepatocellular carcinoma via orthotopic implantation is easy to operate,and has a short operative duration and high reproducibility.It provides an important experimental tool for basic researches and in vivo dynamic monitoring of hepatocellular carcinoma.关键词
肝癌/原位移植瘤/磁共振成像Key words
hepatocellular carcinoma/orthotopic transplantation tumor/magnetic resonance imaging分类
医药卫生引用本文复制引用
应卓鹏,方贾栩,杨小红,战跃福,陈建强..无需缝合外科嵌入式小鼠原位肝癌肿瘤模型的复制及应用价值[J].中国现代医学杂志,2024,34(16):26-32,7.基金项目
海南省自然科学基金面上项目(No:820MS172) （No:820MS172）
海南省重点研发计划项目(No:ZDYF2023SHFZ142) （No:ZDYF2023SHFZ142）
