中国现代医学杂志2024，Vol.34Issue(16)：45-50,6.DOI:10.3969/j.issn.1005-8982.2024.16.008
Th17和调节性T细胞在人类免疫缺陷病毒疾病进展中的作用及其调控机制
Role and regulatory mechanisms of Th17 and regulatory T cells in the progression of HIV infection
摘要
Abstract
Objective To observe the levels of Th17 and Treg cells along with their associated transcription factors and cytokines in peripheral blood of HIV-infected individuals,and to further reveal the regulatory mechanisms of Th17 and Treg cells and their roles in the progression of HIV infection.Methods From January 2019 to October 2019,the 80 HIV-infected individuals from a region in Henan Province were selected as the study group,and 20 healthy people from the same area were included as the control group.The levels of CD4+and CD8+T cells in peripheral blood and Th 17 and Treg cells within peripheral blood mononuclear cells were detected by flow cytometry.The plasma levels of interleukin(IL)-17 and IL-23 were detected by enzyme linked immunosorbent assay.The mRNA expressions of transcription factors ROR-γt and Foxp3 were detected by quantitative real-time polymerase chain reaction.Pearson or Spearman method was used to analyze the correlations between levels of Th17 and Treg cells and the count of CD4+T cells.Results The absolute count of CD4+T cells and ratios of CD4+/CD3+T cells and CD4+/CD8+T cells in the study group were lower than those in the control group(P＜0.05),while the absolute count of CD8+T cells and the ratio of CD8+/CD3+T cells in the study group were higher than those in the control group(P＜0.05).The ratios of Th17/CD4+T cells and Th17/Treg cells in the study group were lower than those in the control group(P＜0.05),whereas the ratio of Treg/CD4+T cells in the study group was higher than that in the control group(P＜0.05).Compared with the control group,the mRNA expression of ROR-γt was higher(P＜0.05),and that of Foxp3 was lower in the study group(P＜0.05).The levels of IL-17 and IL-23 in the study group were lower than those in the control group(P＜0.05).Pearson correlation analysis revealed that the percentage of Th17 cells was positively correlated with the count of CD4+T cells(r=0.293,P＜0.05),and that the percentage of Treg cells was negatively correlated with the count of CD4+T cells(r=-0.198,P＜0.05).Conclusions HIV infection reduces the level of Th17 cells while increasing the level of Treg cells,disrupting the immune balance in the body.The mechanism is related to the regulation of the expressions of transcription factors ROR-γt and Foxp3 and cytokines IL-17 and IL-23.Th17 and Treg cells are closely associated with the disease progression of HIV-infected individuals.关键词
获得性免疫缺陷综合征/人类免疫缺陷病毒/辅助性T细胞17/调节性T细胞/ROR-γt/Foxp3Key words
acquired immunodeficiency syndrome/human immunodeficiency virus/T helper 17 cell/regulatory T cell/ROR-γt/Foxp3分类
医药卫生引用本文复制引用
李延卿,任伟宏,张岱,李文博,张旭冉,桑锋..Th17和调节性T细胞在人类免疫缺陷病毒疾病进展中的作用及其调控机制[J].中国现代医学杂志,2024,34(16):45-50,6.基金项目
河南省科技攻关课题(No:182102310312,No:212102311126) （No:182102310312,No:212102311126）
河南省科技研发计划联合基金(No:232301420080) （No:232301420080）
河南省2023年度河南省"双一流"创建学科中医学科学研究专项(No:HSRP-DFCTCM-2023-1-31) （No:HSRP-DFCTCM-2023-1-31）
