中国现代医学杂志2024，Vol.34Issue(16)：56-61,6.DOI:10.3969/j.issn.1005-8982.2024.16.010
肠道菌群情况与发声和多种运动联合抽动障碍患儿神经递质、神经功能及T细胞亚群的相关性分析
Correlation analysis of gut microbiota with neurotransmitters,neurological functions,and T cell subsets in children with tic disorders
摘要
Abstract
Objective To explore the correlation between the distribution of gut microbiota and neurotransmitters,neurological functions,and T cell subsets in children with tic disorders.Methods A total of 97 children with tic disorders treated in our hospital from May 2020 to May 2023 were selected as research subjects.They were divided into mild(n=23),moderate(n=42),and severe(n=32)groups based on the Yale Global Tic Severity Scale(YGTSS).Fecal samples from all children were collected,and the α-diversity of gut microbiota[including Chaol index,observed species richness(Sobs),Shannon index,and Simpson index]was analyzed using the 16S rRNA sequencing technology and the Qiime software.T lymphocyte subsets(CD4+,CD8+,CD4+/CD8+)and neurotransmitters[5-hydroxytryptamine(5-HT),dopamine(DA),gamma-aminobutyric acid(GABA),and norepinephrine(NE)]were measured.Neurological functions of the two groups of children were assessed.The relationship between gut microbiota and neurotransmitters,neurological functions,and T cell subsets in the children was explored through Pearson correlation analysis.Results Compared to the mild group,the moderate and severe groups had higher motor and vocal tic scores as well as higher total scores as tested via YGTSS(P＜0.05).When comparing the Chaol,Sobs,Shannon,and Simpson indices of children in the mild,moderate,and severe groups,significant statistical differences were found through ANOVA(P＜0.05).Compared with the mild group,the α-diversity in the moderate and severe groups was lower(P＜0.05).Comparisons of the counts of CD4+and CD8+T cells and ratios of CD4+/CD8+T cells among mild,moderate,and severe groups also showed statistically significant differences via variance analysis(P＜0.05).Compared with the mild group,the moderate and severe groups had a lower count of CD4+T cells and a lower ratio of CD4+/CD8+T cells,but a higher count of CD8+T cells(P＜0.05).Compared with the mild group,the moderate and severe groups had higher levels of 5-HT and DA,and lower levels of GABA,NE,and neurological function scores(P＜0.05).According to Pearson correlation analysis,the Chaol,Sobs,Shannon,and Simpson indices were all negatively correlated with the levels of 5-HT and DA(P＜0.05),and positively correlated with the count of CD4+T cells,levels of GABA and NE,and neurological function scores(P＜0.05).Conclusions The gut microbiota are closely related to neurotransmitters,neurological functions,and T cell subsets in children with tic disorders.关键词
肠道菌群多样性/抽动症/神经递质/T细胞亚群/相关性分析Key words
gut microbiota diversity/tic disorders/neurotransmitters/T cell subsets/correlation analysis分类
医药卫生引用本文复制引用
王丹霞,孟珂,尚玉..肠道菌群情况与发声和多种运动联合抽动障碍患儿神经递质、神经功能及T细胞亚群的相关性分析[J].中国现代医学杂志,2024,34(16):56-61,6.基金项目
江苏省自然科学基金(No:BK20210034) （No:BK20210034）
