中国畜牧兽医2024，Vol.51Issue(8)：3247-3255,9.DOI:10.16431/j.cnki.1671-7236.2024.08.003
番茄红素对顺铂所致大鼠脏器损伤的保护效果评价
Evaluation of the Protective Effect of Lycopene on Organ Injury Induced by Cisplatin in Rats
摘要
Abstract
[Objective]The aim of this study was to evaluate the protective effect of lycopene(LCP)on organ injury induced by cisplatin(CDDP)in rats.[Method]Thirty male Wistar rats were randomly divided into 5 groups(n=6),which were control group(CON),CDDP and different LCP treatment groups(5 mg/kg,LLCP group;10 mg/kg,MLCP group;50 mg/kg,HLCP group).Rats in CON and CDDP groups were given 1 mL/kg corn oil per day,and rats in LLCP,MLCP and HLCP groups were given the corresponding concentration of LCP per day,and consecutive for 28 days.Except CON group,the other groups were given a single intraperitoneal injection of CDDP(7 mg/kg)on day 26.After the experiment,samples of stomach,small intestine(duodenum,jejunum and ileum),liver,kidney and blood were collected.The protective effects of different concentrations of LCP on CDDP-induced acute injury in rats were evaluated by physiological and biochemical indexes detection,HE staining,ELISA and Western blotting.[Result]Compared with CON group,body weight,feed intake and water intake of rats in CDDP group were extremely significantly or significantly decreased(P＜0.01 or P＜0.05).Serum levels of aspartate aminotransferase(AST),urea nitrogen(BUN),creatinine(CRE)and malondialdehyde(MDA),interleukin-8(IL-8),IL-6 and tumor necrosis factor-α(TNF-α)were significantly or extremely significantly increased(P＜0.05 or P＜0.01).The activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)were extremely significantly decreased(P＜0.01),and the histopathological injuries of stomach,small intestine,liver and kidney were serious.Compared with CDDP group,the body weight,feed intake and water intake of rats in MLCP group were significantly increased(P＜0.05),the levels of 6 biochemical indexes such as AST and ALT and the contents of MDA,IL-8,TNF-α and IL-6 in serum were significantly decreased(P＜0.05),and the organ histomathological damage was alleviated.Western blotting results showed that compared with CON group,the expression of TNF-α and IL-6 proteins in different tissues of rats in CDDP group were extremely significantly increased(P＜0.01),while the expression of IL-10 protein was extremely significantly decreased(P＜0.01).Compared with CDDP group,the expression of TNF-α and IL-6 proteins in all tissues of MLCP group were extremely significantly or significantly decreased(P＜0.01 or P＜0.05),the expression of IL-10 protein was extremely significantly or significantly increased(P＜0.01 or P＜0.05).[Conclusion]LCP could improve the stomach,small intestine,liver and kidney injury in CDDP-induced rats by inhibiting oxidative stress and inflammation.Under this experiment conditions,10 mg/kg LCP had the best effect.关键词
番茄红素/顺铂/大鼠/脏器损伤/炎症Key words
lycopene/cisplatin/rat/organ injury/inflammation分类
农业科技引用本文复制引用
孙悦,田雪,杨春雪,徐恩爽,郑家三..番茄红素对顺铂所致大鼠脏器损伤的保护效果评价[J].中国畜牧兽医,2024,51(8):3247-3255,9.基金项目
黑龙江省省属高等学校基本科研业务费(TDJH201903-3) （TDJH201903-3）
