诃子提取物对脂多糖刺激肉鸡肠道和肝脏损伤的影响OA北大核心CSTPCD

[Objective]This study was conducted to investigate the effect of chebuli extract(CE)on the intestinal morphology,intestinal and liver inflammatory responses of lipopolysaccharide(LPS)-stimulated broilers,so as to provide a theoretical basis for the development of functional feed additives.[Method]This experiment adopted a two-factor experimental design.40 healthy 1-day-old Ross 308 broilers were selected and randomly divided into control and CE treatment groups.Each group had 5 replicates with 4 broilers in each replicate.The broilers in control group were fed basal diet,and broilers in CE treatment group was supplemented with 1 000 mg/kg CE on the basis of basal diet.The experimental period was 29 days.On the 29th day,2 broilers in each replicate were intraperitoneally injected with 1 mg/kg BW LPS,and the other 2 broilers were intraperitoneally injected with normal saline.Slaughter sampling was performed 3 h after LPS injection.The liver,spleen,thymus and bursa of Fabricius of broilers were weighed to calculate organ coefficients.Intestinal tissue morphology,intestinal barrier-related genes,and intestinal and liver inflammation-related genes of broilers were measured.[Result]① Compared with broilers without LPS stimulation,LPS stimulation significantly reduced villus height,crypt depth,villus height/crypt depth and villus area of duodenum,and jejunum villus height and ileum villus area in broilers(P＜0.05);Adding CE significantly reduced the jejunum crypt depth,and significantly increased villus height,villus height/crypt depth and villus area of jejunum and ileum(P＜0.05).Compared with LPS-stimulated broilers,adding CE significantly increased villus height of duodenum,jejunum and ileum,villus height/crypt depth of duodenum and ileum,and villus area of jejunum and ileum(P＜0.05).② Compared with broilers without LPS stimulation,adding CE significantly increased the expression of Mucin-2,zonula occludin 1(ZO-1)and Occludin genes in broilers(P＜0.05).Compared with LPS-stimulated broilers,adding CE significantly increased the expression of Claudin-1,Mucin-2 and ZO-1 genes in broilers(P＜0.05).③ Compared with broilers without LPS stimulation,LPS stimulation significantly increased the expression of matrix metalloproteinase 9(MMP9),interleukin-1β(IL-1β),IL-8 and tumor necrosis factor-α(TNF-α)genes in liver of broilers(P＜0.05);Adding CE significantly reduced the expression of MMP9,IL-1β and IL-8 genes in liver of broilers(P＜0.05).Compared with LPS-stimulated broilers,adding CE significantly decreased the expression of MMP9 and interferon-γ(IFN-γ)genes in liver of broilers(P＜0.05).[Conclusion]CE improved the intestinal morphology and structure of LPS-stimulated broilers,enhanced the intestinal barrier function,and mitigated the inflammatory response in liver of LPS-stimulated broilers.The results provided a theoretical basis for the application of CE as a natural feed additive.