中国畜牧兽医2024，Vol.51Issue(8)：3355-3364,10.DOI:10.16431/j.cnki.1671-7236.2024.08.014
母马补喂支链氨基酸对哺乳马驹生长性能和粪便菌群的影响
Effects of Supplemental Feeding of Branched-chain Amino Acids to Mares on Growth Performance and Fecal Flora of Lactating Foals
摘要
Abstract
[Objective]The aim of this experiment was to investigate the effects of supplemental feeding of different doses of branched-chain amino acids(BCAAs)to Yili mares during lactation on the growth performance and fecal flora of lactating foals,in order to provide a reference basis for safeguarding the growth and health of foals during lactation.[Method]Twenty lactating mares and their foals(lactating foals between 2 and 3 months of age,with an initial weight of 97.60 kg±13.24 kg,lactating mares weighing 392.90 kg±12.18 kg)were selected and randomly divided into four groups of five mares each,the control group and the experimental groups Ⅰ,Ⅱ,and Ⅲ,and were kept under the same feeding management conditions for 67 d(7 d pre-feeding period,60 d experimental period).During the test period,mares in control and test groups were supplemented with 2 kg of concentrate per day,and mares in test groups Ⅰ,Ⅱ,and Ⅲ were supplemented with 38,76 and 114 g/d of BCAAs,respectively.Body measurements and body weights were taken on the day before experiment and 60th day of the experiment,and feces were collected by rectal fecal sampling on the 60th day,and fecal flora diversity of foals was analyzed.[Result]On the 60th day of the trial,the breast circumference and body weight of foals in the test group Ⅲ were significantly higher than those in control group(P＜0.05),and the body weight of foals in the test group Ⅱ was significantly higher than those in control group(P＜0.05).The number of fecal flora OTUs was 4 553,4 483,4 607 and 3 839 in test groups Ⅰ,Ⅱ,Ⅲ,and control groups,respectively.There was no significant difference in the Alpha diversity index as well as the number of species observed among the groups(P＞0.05).The relative abundance of Spirochaetota in test group Ⅱ was significantly higher than that in control group and test groupⅠ(P＜0.05),and the relative abundance of Lachnospiraceae was significantly higher than that of control and test group Ⅲ(P＜0.05).The relative abundance of Rumenococcaceae was significantly higher in both test groups Ⅰ and Ⅱ than control group(P＜0.05),and the relative abundance of Rikenellaceae in test group Ⅲ was significantly higher than that in control group(P＜0.05).At the genus level,the relative abundance of Treponema was the highest and significantly higher in test group Ⅱ than that in test group Ⅰ(P＜0.05),and the relative abundance of Ruminococcus was significantly higher than in the other three groups(P＜0.05).The relative abundance of Prevotellaceae_UCG_004 in test group Ⅲ was highly significantly higher than that of control group and test group Ⅰ(P＜0.01).The differential items exhibited by LEfSe analysis and PICRUSt2 function prediction were all related to intestinal fermentation metabolism,short-chain fatty acid production,and amino acid metabolism.[Conclusion]Supplemental feeding of different doses of BCAAs to Yili mares could promote the growth of breast circumference and body weight of lactating foals,increase the abundance of beneficial intestinal flora,and decrease the abundance of harmful flora,and the best results were obtained by supplementing mares with 76 g/d of BCAAs.关键词
母马/支链氨基酸/马驹/粪便菌群Key words
mare/branched-chain amino acids/foal/fecal flora分类
农业科技引用本文复制引用
刘笑天,贾雨欣,高凤,袁鑫鑫,王永发,薛宇恒,孟军,曾亚琦,李晓斌..母马补喂支链氨基酸对哺乳马驹生长性能和粪便菌群的影响[J].中国畜牧兽医,2024,51(8):3355-3364,10.基金项目
新疆维吾尔自治区重大科技专项"国产马专门化品系高效繁育技术体系构建"(2022A02013-1) （2022A02013-1）
新疆维吾尔自治区创新环境(人才、基地)建设专项"马种质创新培育与高效健康养殖重点实验室平台建设"(PT2311) （人才、基地）
中央引导地方科技发展专项资金项目"专门化运动马培育技术服务支撑"(ZYYD2023C02) （ZYYD2023C02）
