中国药科大学学报2024，Vol.55Issue(4)：432-442,11.DOI:10.11665/j.issn.1000-5048.2024040202
Ⅱ型鼠李半乳糖醛酸聚糖的制备方法和结构表征的研究进展
Advances in the preparation and structural characterization of rhamnogalacturonan Ⅱ
摘要
Abstract
Rhamnogalacturonan Ⅱ(RG-Ⅱ)is one of the structural domains of pectin whose structure is highly conserved among species.The main chain of RG-Ⅱ consists of approximately nine galacturonic acids linked by α-1,4 glycosidic bonds,with six well-defined side chains replacing them(A-F).The structures of the disaccharide side chains C and D and the monosaccharide side chains E and F in RG-Ⅱ from different sources remain essentially the same.In contrast,the oligosaccharide side chains A and B showed slight variability.Structural characterization of RG-Ⅱ can be achieved by molecular weight,monosaccharide composition,and mass spectrometry.The polysaccharides containing RG-Ⅱ structural domains in traditional Chinese medicines(TCMs)have high medicinal value.Isolation of RG-Ⅱ can be achieved using endo-polygalacturonase(Endo-PG)and Penicillium oxalicum.A substantial number of RG-Ⅱ standards can be rapidly prepared from red wine for the development of new quantitative methods to realize the quality control of active polysaccharides from traditional Chinese medicines and to promote the research process of polysaccharides from traditional Chinese medicines.关键词
果胶/Ⅱ型鼠李半乳糖醛酸聚糖/RG-Ⅱ/内切多聚半乳糖醛酸酶/草酸青霉/葡萄酒Key words
pectin/rhamnogalacturonan Ⅱ/RG-Ⅱ/endo-polygalacturonase/penicillium oxalate/red wine分类
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刘育朋,施松善,步盈萱,龚欢,王辉俊,王顺春..Ⅱ型鼠李半乳糖醛酸聚糖的制备方法和结构表征的研究进展[J].中国药科大学学报,2024,55(4):432-442,11.基金项目
上海市自然科学基金项目(No.21ZR1462900),国家自然科学基金项目(No.82274078,No.U23A20512),上海市东方英才计划青年项目 This study was supported by the Natural Science Foundation of Shanghai(No.21ZR1462900),the National Natural Science Foundation of China(No.82274078,No.U23A20512),and Shanghai Oriental Talent Program for Youth （No.21ZR1462900）
