中国药科大学学报2024，Vol.55Issue(4)：472-477,6.DOI:10.11665/j.issn.1000-5048.2024020502
单一内标定量核磁法测定肺炎链球菌多糖抗原中荚膜多糖、C-多糖和磷含量
Quantitative determination of capsular polysaccharide,C-polysaccharide,phosphorus of carbohydrate antigens from Streptococcus pneumoniae by quantitative NMR using a single internal standard
摘要
Abstract
Pneumococcal vaccine plays a key role in preventing diseases such as pneumonia and meningitis caused by Streptococcus pneumoniae(S.pneumoniae).Capsular polysaccharide,C-polysaccharide and phosphorus content are important indicators for quality control of polysaccharide antigens in vaccine development and production.In this study,a quantitative 1H NMR and 31P NMR method based on a single internal standard hexamethylphosphoramide(HMPA)was developed to achieve simultaneous determination of capsular polysaccharide,C-polysaccharide and phosphorus content in 6A,6B,18C,19A,19F and 23F S.pneumoniae polysaccharide antigens.Using the internal reference comparison method,the effect of solubility of polysaccharide on quantitative 1H NMR determination was investigated.It was found that the determination results of quantitative 1H NMR were affected by the viscosity and concentration of polysaccharide solution.It was found that high viscosity polysaccharides at 3-15 mg/mL and low viscosity polysaccharides at 5-25 mg/mL were the optimal detection solution concentration range.This"one internal standard three quantitative"NMR method has good precision,specificity and accuracy,and provides a valuable new strategy for the quality control of pneumococcal vaccine.关键词
肺炎链球菌/荚膜多糖/定量1H NMR/定量31P NMR/C-多糖/磷Key words
Streptococcus pneumoniae/capsular polysaccharide/quantitative 1H NMR/quantitative 31P NMR/C-polysaccharide/phosphorus分类
医药卫生引用本文复制引用
郭吉茹,秦春君,胡静,曹欣,徐永学,刘建凯,尹健..单一内标定量核磁法测定肺炎链球菌多糖抗原中荚膜多糖、C-多糖和磷含量[J].中国药科大学学报,2024,55(4):472-477,6.基金项目
国家自然科学基金项目(No.22325803,No.22277042,No.22077052,No.22177041) This study was supported by the National Natural Science Foundation of China(No.22325803,No.22277042,No.22077052,No.22177041) （No.22325803,No.22277042,No.22077052,No.22177041）
